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PMID: 3537805 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes.

Nature ·Vol. 324 ·No. 6095 ·1986-00-00 ·Pages 341-5

Pikielny CW, Rymond BC, Rosbash M

Abstract

Three splicing complexes formed with a yeast pre-messenger RNA during in vitro splicing can be resolved by non-denaturing gel electrophoresis after incubation in the presence of non-specific competitor RNA. The time course of the appearance of these complexes and their composition suggest that they represent an ordered pathway of splicing complex assembly.

MeSH Terms

Electrophoresis, Polyacrylamide Gel/methods Macromolecular Substances Nucleic Acid Precursors/isolation & purification RNA Precursors RNA Splicing RNA, Messenger/isolation & purification RNA, Small Nuclear/isolation & purification Ribonucleoproteins/genetics,isolation & purification Saccharomyces cerevisiae/genetics

Chemicals

Macromolecular Substances Nucleic Acid Precursors RNA Precursors RNA, Messenger RNA, Small Nuclear Ribonucleoproteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Pikielny C W

Rymond B C

Rosbash M

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/324341a0

Published

1986-00-00

Pages

341-5

Language

English

Region

England

NLM ID

0410462

Subset

Grants

NIGMS NIH HHS · GM23549 · United States

External Links

PubMed source DOI: 10.1038/324341a0 View journal details

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Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes.

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