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PMID: 3539802 Published · ppublish English Journal Article

Presence and expression of aerobactin genes in virulent avian strains of Escherichia coli.

Infection and immunity ·Vol. 55 ·No. 1 ·1987-01-00 ·Pages 193-7

Lafont JP, Dho M, D'Hauteville HM, Bree A, Sansonetti PJ

Abstract

Virulent and nonvirulent isolates of avian Escherichia coli were tested for the presence of aerobactin genes by colony hybridization with a specific gene probe constructed from plasmid pABN1 (A. Bindereif and J. B. Neilands, J. Bacteriol. 153:1111-1113, 1983). Positive hybridization with the gene probe was highly correlated with virulence, as measured by the 50% lethal dose of the strains for chicks. Evidence for the expression of aerobactin genes in the virulent strains was obtained by demonstrating their susceptibility to cloacin DF13, which binds to the same receptor that binds aerobactin, and their ability to produce aerobactin, as revealed by cross-feeding the E. coli mutant WO987 (aroB fepA iuc iut+), which is unable to synthesize but capable of taking up aerobactin. We suggest that the production of aerobactin is involved in the virulence of avian septicemic E. coli.

MeSH Terms
Animals Chickens Cloacin/pharmacology DNA, Bacterial/genetics Escherichia coli/genetics,pathogenicity Gene Expression Regulation Genes, Bacterial Hydroxamic Acids/physiology Transferrin/pharmacology
Chemicals
DNA, Bacterial Hydroxamic Acids Transferrin aerobactin Cloacin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lafont J P
Dho M
D'Hauteville H M
Bree A
Sansonetti P J
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30 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.1.193-197.1987
Published
1987-01-00
Pages
193-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260300
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.55.1.193-197.1987 PMC Full Text View journal details
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