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PMID: 3540600 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins.

Molecular and cellular biology ·Vol. 6 ·No. 11 ·1986-11-00 ·Pages 3722-33

Schatz PJ, Solomon F, Botstein D

Abstract

Microtubules in yeast are essential components of the mitotic and meiotic spindles and are essential for nuclear movement during cell division and mating. The relative importance in these processes of the two divergent alpha-tubulin genes of the budding yeast Saccharomyces cerevisiae, TUB1 and TUB3, was examined through the construction of null mutations and by increasing their copy number on chromosomes and on plasmids. Experiments with null alleles of TUB3 showed that TUB3 was not essential for mitosis, meiosis, or mating. Null alleles of TUB3, however, did cause several phenotypes, including hypersensitivity to the antimicrotubule drug benomyl and poor spore viability. On the other hand, the TUB1 gene was essential for growth of normal haploid cells. Even in diploids heterozygous for a TUB1 null allele, several dominant phenotypes were evident, including slow growth and poor sporulation. This functional difference between the two genes is apparently due to different levels of expression, because extra copies of either gene could suppress the defects caused by a null mutation in the other. We conclude that in spite of the 10% divergence between the products of the two genes, there is no essential qualitative functional difference between them.

MeSH Terms
Alleles Chromosome Mapping Crosses, Genetic Genes Genes, Fungal Genetic Complementation Test Genetic Linkage Genotype Plasmids Saccharomyces cerevisiae/genetics Suppression, Genetic Tubulin/genetics
Chemicals
Tubulin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schatz P J
Solomon F
Botstein D
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55 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.11.3722-3733.1986
Published
1986-11-00
Pages
3722-33
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367133
Subset
IM
Grants
NIGMS NIH HHS · GM-18973 · United States
NIGMS NIH HHS · GM-21253 · United States
NCI NIH HHS · P01-CA-26712 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.11.3722-3733.1986 PMC Full Text View journal details
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