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PMID: 3540936 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Promoter domain mediates guanosine tetraphosphate activation of the histidine operon.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 24 ·1986-12-00 ·Pages 9333-7

Riggs DL, Mueller RD, Kwan HS, Artz SW

Abstract

We have analyzed the effects of the "alarmone" guanosine 5'-diphosphate 3'-diphosphate (ppGpp) on regulation of the Salmonella typhimurium histidine operon in vitro. Expression of the wild-type promoter, measured in a DNA-dependent transcription-translation system, was strongly dependent on ppGpp; addition of ppGpp stimulated his expression 22-fold with plasmid DNA templates. Oligonucleotide-directed, site-specific mutations that increase the homology of the -10 hexamer to the consensus sequence of the E sigma 70 promoters dramatically increased his expression in the absence of ppGpp and reduced the stimulation to less than a factor of 2. A deletion mutation that alters the sequence between the -10 hexamer and the start point of transcription, generated by BAL-31 nuclease, affected ppGpp regulation in a similar manner. We propose that the -10 hexamer sequence and the adjacent downstream region are both important in regulating transcription by ppGpp. Mechanisms to account for activation and repression of transcription by ppGpp are discussed.

MeSH Terms
DNA, Bacterial/genetics DNA, Superhelical/genetics Gene Expression Regulation Guanine Nucleotides/pharmacology Guanosine Tetraphosphate/pharmacology Histidine/genetics Operon Promoter Regions, Genetic Salmonella typhimurium/genetics Transcription, Genetic/drug effects
Chemicals
DNA, Bacterial DNA, Superhelical Guanine Nucleotides Guanosine Tetraphosphate Histidine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Riggs D L
Mueller R D
Kwan H S
Artz S W
References (45)
45 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.24.9333
Published
1986-12-00
Pages
9333-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC387132
Subset
IM
Grants
NIGMS NIH HHS · GM07377 · United States
NIGMS NIH HHS · GM27307 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.24.9333 PMC Full Text View journal details
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