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PMID: 3542834 Published · ppublish English Journal Article

Expression of staphylococcal enterotoxin C1 in Escherichia coli.

Infection and immunity ·Vol. 55 ·No. 2 ·1987-02-00 ·Pages 428-32

Bohach GA, Schlievert PM

Abstract

The structural gene encoding staphylococcal enterotoxin C1 was cloned into Escherichia coli and localized on a 1.5-kilobase HindIII-ClaI DNA fragment by subcloning. The toxin was partially purified from E. coli clones and shown to be immunologically identical to enterotoxin C1 from Staphylococcus aureus. The cloned toxin also had the same molecular weight (26,000) and charge heterogeneity as staphylococcus-derived enterotoxin. Toxins from both sources were equally biologically active.

MeSH Terms
Animals Cloning, Molecular Enterotoxins/genetics,toxicity Escherichia coli/genetics Mice Molecular Weight Plasmids Rabbits
Chemicals
Enterotoxins enterotoxin C, staphylococcal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bohach G A
Schlievert P M
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33 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.2.428-432.1987
Published
1987-02-00
Pages
428-32
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260345
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.55.2.428-432.1987 PMC Full Text View journal details
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