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PMID: 3543621 Published · ppublish English Journal Article

Alteration by mutation of the control by oxygen of the nar operon in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 205 ·No. 2 ·1986-11-00 ·Pages 349-52

Bonnefoy V, Pascal MC, Ratouchniak J, Chippaux M

Abstract

A nar-lac operon fusion was used to isolate a mutant in which the expression of the nar operon was no longer repressed by oxygen. The nard mutation, located upstream of the nar structural genes, was found to be cis dominant; it led to independence from the Fnr protein which, in the wild-type strain, exerts a strict positive control on the nar operon. Both other known controls, nitrate induction and autoregulation, were unaffected. It is proposed that molecular oxygen controls the expression of nar via Fnr and that the nard mutation affects the Fnr binding site of the narGHI control region.

MeSH Terms
Aerobiosis Anaerobiosis Conjugation, Genetic Escherichia coli/genetics Genes, Bacterial Genotype Mutation Operon Oxygen Consumption Phenotype Transduction, Genetic
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bonnefoy V
Pascal M C
Ratouchniak J
Chippaux M
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23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00430449
Published
1986-11-00
Pages
349-52
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00430449
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