Home LiteratureArticle Details
PMID: 3547405 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 5 ·1987-03-00 ·Pages 1127-31

Recsei PA, Gruss AD, Novick RP

Abstract

A 1.5-kilobase-pair fragment of DNA that contains the lysostaphin gene from Staphylococcus simulans and its flanking sequences has been cloned and completely sequenced. The gene encodes a preproenzyme of Mr 42,000. The NH2-terminal sequence of the preproenzyme is composed of a signal peptide followed by seven tandem repeats of a 13-amino acid sequence. Conversion of prolysostaphin to the mature enzyme occurs extracellularly in cultures of S. simulans and involves removal of the NH2-terminal portion of the proenzyme that contains the tandem repeats. The high degree of homology of the repeats suggests that they have arisen by duplication of a 39-base-pair sequence of DNA. In S. simulans, the lysostaphin gene is present on a large beta-lactamase plasmid.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Escherichia coli/drug effects Genes Genes, Bacterial Lysostaphin/genetics,pharmacology Staphylococcus/genetics Transcription, Genetic
Chemicals
Lysostaphin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Recsei P A
Gruss A D
Novick R P
References (43)
43 references, click to expand
  1. Histidine decarboxylase of Lactobacillus 30a. Comparative properties of wild type and mutant proenzymes and their derived enzymes.
    J Biol Chem. 1982 Jun 25;257(12):7196-202 PMID: 7085622
  2. LYSOSTAPHIN: A NEW BACTERIOLYTIC AGENT FOR THE STAPHYLOCOCCUS.
    Proc Natl Acad Sci U S A. 1964 Mar;51:414-21 PMID: 14171453
  3. Colicins and other bacteriocins with established modes of action.
    Annu Rev Microbiol. 1982;36:125-44 PMID: 6184011
  4. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  5. Phylogeny of the conserved 3' terminal structure of the RNA of small ribosomal subunits.
    Nucleic Acids Res. 1984 Mar 26;12(6):2595-604 PMID: 6709501
  6. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  7. A rapid alkaline extraction method for the isolation of plasmid DNA.
    Methods Enzymol. 1983;100:243-55 PMID: 6353143
  8. Mutagenesis of extrachromosomal genetic determinants for exfoliative toxin B and bacteriocin R1 synthesis in Staphylococcus aureus after plasmid transfer by protoplast fusion.
    Infect Immun. 1983 Dec;42(3):973-9 PMID: 6642673
  9. Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis.
    Nucleic Acids Res. 1983 Nov 25;11(22):7911-25 PMID: 6316278
  10. Complete sequence of the staphylococcal gene encoding protein A. A gene evolved through multiple duplications.
    J Biol Chem. 1984 Feb 10;259(3):1695-702 PMID: 6319407
  11. The subtilisin E gene of Bacillus subtilis is transcribed from a sigma 37 promoter in vivo.
    Proc Natl Acad Sci U S A. 1984 Feb;81(4):1184-8 PMID: 6322190
  12. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  13. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  14. Molecular properties of lysostaphin, a bacteriolytic agent specific for Staphylococcus aureus.
    J Biol Chem. 1970 Sep 25;245(18):4842-6 PMID: 5456157
  15. Bacterial genome sizes determined by DNA renaturation studies.
    J Gen Microbiol. 1970 Dec;64(3):377-80 PMID: 4396963
  16. Studies on plasmid replication. I. Plasmid incompatibility and establishment in Staphylococcus aureus.
    J Mol Biol. 1972 Jul 21;68(2):285-302 PMID: 4262657
  17. Studies on lysostaphin. Separation and characterization of three enzymes.
    Eur J Biochem. 1973 Oct 5;38(2):293-300 PMID: 4773876
  18. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  19. Simplified scheme for routine identification of human Staphylococcus species.
    J Clin Microbiol. 1975 Jan;1(1):82-8 PMID: 170303
  20. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  21. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.
    Cell. 1976 Sep;9(1):91-9 PMID: 788919
  22. The immunochemistry of peptidoglycan. Antibodies against a synthetic immunogen cross-reacting with an interpeptide bridge of peptidoglycan.
    Eur J Biochem. 1977 Apr 1;74(2):353-63 PMID: 856579
  23. Modification of tryptophan to oxindolylalanine by dimethyl sulfoxide-hydrochloric acid.
    Methods Enzymol. 1977;47:442-53 PMID: 927197
  24. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  25. Relationship between lysostaphin endopeptidase production and cell wall composition in Staphylococcus staphylolyticus.
    J Bacteriol. 1979 Mar;137(3):1158-64 PMID: 438117
  26. Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps.
    Plasmid. 1979 Jan;2(1):109-29 PMID: 314115
  27. Rapid separation of amino acid phenylthiohydantoins by isocratic high-performance liquid chromatography.
    Anal Biochem. 1981 Feb;111(1):27-32 PMID: 7235237
  28. Lysostaphin disk test for routine presumptive identification of staphylococci.
    J Clin Microbiol. 1981 Jun;13(6):1023-5 PMID: 7019233
  29. Methods for the preparation of enzyme-antibody conjugates for use in enzyme immunoassay.
    Methods Enzymol. 1981;73(Pt B):147-66 PMID: 6272056
  30. Plasmids in epidermolytic strains of Staphylococcus aureus.
    J Gen Microbiol. 1981 May;124(1):99-107 PMID: 6275008
  31. High-level potentiation of lysostaphin anti-staphylococcal activity by lysozyme.
    Antimicrob Agents Chemother. 1982 Apr;21(4):531-5 PMID: 7081976
  32. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.
    Anal Biochem. 1984 Jan;136(1):175-9 PMID: 6424501
  33. Replacement of the Bacillus subtilis subtilisin structural gene with an In vitro-derived deletion mutation.
    J Bacteriol. 1984 May;158(2):411-8 PMID: 6427178
  34. Compilation of published signal sequences.
    Nucleic Acids Res. 1984 Jul 11;12(13):5145-64 PMID: 6379599
  35. Sensory transduction in Escherichia coli: regulation of the demethylation rate by the CheA protein.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5061-5 PMID: 6382257
  36. Staphylococcal enterotoxin A gene is associated with a variable genetic element.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5179-83 PMID: 6089183
  37. Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein.
    J Bacteriol. 1984 Sep;159(3):811-9 PMID: 6090391
  38. Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation.
    J Bacteriol. 1984 Oct;160(1):15-21 PMID: 6090407
  39. Processing of Bacillus cereus 569/H beta-lactamase I in Escherichia coli and Bacillus subtilis.
    J Biol Chem. 1985 Jan 25;260(2):1218-23 PMID: 3918030
  40. Plasmid involvement in production of and immunity to the staphylococcin-like peptide Pep 5.
    J Gen Microbiol. 1984 Nov;130(11):3029-35 PMID: 6527128
  41. Complete nucleotide sequence of type 6 M protein of the group A Streptococcus. Repetitive structure and membrane anchor.
    J Biol Chem. 1986 Feb 5;261(4):1677-86 PMID: 3511046
  42. Penicillinase plasmids of Staphylococcus aureus: structural and evolutionary relationships.
    Plasmid. 1980 May;3(3):291-311 PMID: 6100898
  43. Coordinate production of three exoenzymes of Staphylococcus staphylolyticus.
    J Gen Microbiol. 1982 Jul;128(7):1529-35 PMID: 6750034
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.5.1127
Published
1987-03-00
Pages
1127-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304379
Subset
IM
Grants
NIGMS NIH HHS · GM-14372 · United States
Databases
GENBANK
M15686
External Links
PubMed source DOI: 10.1073/pnas.84.5.1127 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2