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PMID: 3550698 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Messenger RNA stability in Saccharomyces cerevisiae: the influence of translation and poly(A) tail length.

Nucleic acids research ·Vol. 15 ·No. 6 ·1987-03-25 ·Pages 2417-29

Santiago TC, Bettany AJ, Purvis IJ, Brown AJ

Abstract

A comparison between the half-lives of 10 specific yeast mRNAs and their distribution within polysomes (fractionated on sucrose density gradients) was used to test the relationship between mRNA translation and degradation in the eukaryote Saccharomyces cerevisiae. Although the mRNAs vary in their distribution across the same polysome gradients, there is no obvious correlation between the stability of an mRNA and the number of ribosomes it carries in vivo. This suggests that ribosomal protection against nucleolytic attack is not a major factor in determining the stability of an mRNA in yeast. The relative lengths of the poly(A) tails of 9 yeast mRNAs were analysed using thermal elution from poly(U)-Sepharose. No dramatic differences in poly(A) tail length were observed amongst the mRNAs which could account for their wide ranging half-lives. Minor differences were consistent with shortening of the poly(A) tail as an mRNA ages.

MeSH Terms
Half-Life Nucleic Acid Hybridization Plasmids Poly A/genetics Polyribosomes/metabolism Protein Biosynthesis RNA, Messenger/genetics,metabolism Saccharomyces cerevisiae/genetics
Chemicals
RNA, Messenger Poly A
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Santiago T C
Bettany A J
Purvis I J
Brown A J
References (39)
39 references, click to expand
  1. Regulation of protein synthesis during heat shock.
    Nature. 1981 Sep 24;293(5830):311-4 PMID: 6792546
  2. The effect of age on the properties of poly(A)-containing messenger RNA in Physarum polycephalum.
    J Gen Microbiol. 1981 Jan;122(1):143-50 PMID: 6119350
  3. A new nonsense mutation as the molecular basis for beta thalassaemia.
    J Mol Biol. 1982 Jan 25;154(3):537-40 PMID: 6896219
  4. lac Transcription in Escherichia coli cells treated with chloramphenicol.
    J Bacteriol. 1982 Jul;151(1):251-61 PMID: 7045075
  5. The regulatory function of poly(A) and adjacent 3' sequences in translated RNA.
    Prog Nucleic Acid Res Mol Biol. 1982;27:53-83 PMID: 7048421
  6. The isolation, characterization, and sequence of the pyruvate kinase gene of Saccharomyces cerevisiae.
    J Biol Chem. 1983 Feb 25;258(4):2193-201 PMID: 6185493
  7. In vivo transcription of a eukaryotic regulatory gene.
    EMBO J. 1983;2(12):2179-84 PMID: 6321151
  8. Mutationally altered 3' ends of yeast CYC1 mRNA affect transcript stability and translational efficiency.
    J Mol Biol. 1984 Jul 25;177(1):107-35 PMID: 6086937
  9. Regulation of messenger RNA stability in mouse erythroleukemia cells.
    J Mol Biol. 1985 Jan 20;181(2):231-9 PMID: 3856689
  10. Truncation of exon 1 from the c-myc gene results in prolonged c-myc mRNa stability.
    EMBO J. 1985 Dec 30;4(13B):3727-33 PMID: 4092694
  11. Identification of a positive retroregulator that stabilizes mRNAs in bacteria.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3233-7 PMID: 3085085
  12. The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments.
    Cell. 1986 Jul 18;46(2):245-51 PMID: 3521892
  13. A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
    Cell. 1986 Aug 29;46(5):659-67 PMID: 3488815
  14. A mutation allowing an mRNA secondary structure diminishes translation of Saccharomyces cerevisiae iso-1-cytochrome c.
    Mol Cell Biol. 1985 Aug;5(8):1839-46 PMID: 3018530
  15. The relationship between mRNA stability and length in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1986 Nov 11;14(21):8347-60 PMID: 3537957
  16. Macromolecule synthesis in yeast spheroplasts.
    J Bacteriol. 1967 Nov;94(5):1697-705 PMID: 6066050
  17. The dissociation of rat liver ribosomes by ethylenediaminetetraacetic acid; molecular weights, chemical composition, and buoyant densities of the subunits.
    Biochemistry. 1969 Mar;8(3):851-6 PMID: 4976408
  18. Polarity and the degradation of mRNA.
    Nature. 1969 Oct 25;224(5217):329-31 PMID: 4898925
  19. Polarity induced by chloramphenicol and relief by suA.
    J Mol Biol. 1971 Jan 14;55(1):113-8 PMID: 4926972
  20. Hyper-labile messenger RNA in polar mutants of the tryptophan operon of Escherichia coli.
    J Mol Biol. 1972 Dec 14;72(1):103-10 PMID: 4567396
  21. Polyadenylic acid sequences in yeast messenger ribonucleic acid.
    J Biol Chem. 1973 Feb 25;248(4):1466-71 PMID: 4568819
  22. Diversity of regulation of genetic transcription. I. Effect of antibiotics which inhibit the process of translation on RNA metabolism in Escherichia coli.
    J Mol Biol. 1973 Feb 25;74(2):113-36 PMID: 4570287
  23. Possible relationship of poly(A) shortening to mRNA turnover.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1077-81 PMID: 1055367
  24. Coupling of rates of transcription, translation, and messenger ribonucleic acid degradation in streptomycin-dependent mutants of Escherichia coli.
    J Bacteriol. 1976 Jan;125(1):84-93 PMID: 1107319
  25. Methylated nucleosides in polyadenylate-containing yeast messenger ribonucleic acid.
    Biochim Biophys Acta. 1976 Apr 2;425(4):401-8 PMID: 769826
  26. Methylated, blocked 5' termini of yeast mRNA.
    J Biol Chem. 1976 May 25;251(10):2898-904 PMID: 773935
  27. Globin mRNA species containing poly(A) segments of different lengths. Their functional stability in Xenopus oocytes.
    Eur J Biochem. 1976 Apr 15;64(1):115-21 PMID: 776610
  28. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
  29. Translation and mRNA decay.
    Mol Gen Genet. 1978 Apr 6;160(2):121-9 PMID: 349350
  30. Transformation of yeast by a replicating hybrid plasmid.
    Nature. 1978 Sep 14;275(5676):104-9 PMID: 357984
  31. A correlation between the rate of poly(A) shortening and half-life of messenger RNA in adenovirus transformed cells.
    J Mol Biol. 1978 Nov 25;126(1):23-36 PMID: 739542
  32. Individual messenger RNA half lives in Saccharomyces cerevisiae.
    Mol Gen Genet. 1979 Feb 26;170(2):129-35 PMID: 372757
  33. The half-life of mRNA in Saccharomyces cerevisiae.
    Mol Gen Genet. 1979 Feb 26;170(2):137-44 PMID: 372758
  34. Fractionation and functional analysis of newly synthesized and decaying messenger RNAs from vegetative cells of Dictyostelium discoideum.
    J Mol Biol. 1979 Mar 5;128(3):371-95 PMID: 439140
  35. beta 0 thalassemia, a nonsense mutation in man.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2886-9 PMID: 88735
  36. Interference of nonsense mutations with eukaryotic messenger RNA stability.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5134-7 PMID: 388431
  37. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  38. Utilization of polyadenylate mRNA during growth and starvation in Physarum polycephalum.
    Eur J Biochem. 1980 Sep;110(2):413-20 PMID: 6108214
  39. Yeast histone mRNA is polyadenylated.
    Nucleic Acids Res. 1980 Dec 11;8(23):5725-37 PMID: 7008028
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.6.2417
Published
1987-03-25
Pages
2417-29
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340660
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.6.2417 PMC Full Text View journal details
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