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PMID: 3553151 Published · ppublish English Journal Article

Cloning and nucleotide sequence of the chlD locus.

Journal of bacteriology ·Vol. 169 ·No. 5 ·1987-05-00 ·Pages 1911-6

Johann S, Hinton SM

Abstract

The nucleotide sequence of a Sau3A1 restriction nuclease fragment that complemented an Escherichia coli chlD::Mu cts mutant strain was determined. DNA and deduced amino acid sequence analysis revealed two open reading frames (ORFs) that potentially codes for proteins with amino acid sequence homology with binding protein-dependent transport systems. One of the ORFs showed a sequence that encoded a protein with properties that were characteristic of a hydrophobic inner membrane protein. The other ORF, which was responsible for complementing a chlD mutant, encoded a protein with conserved sequences in nucleotide-binding proteins and hydrophilic inner membrane proteins in active transport systems. A proposal that the chlD locus is the molybdate transport operon is discussed in terms of the chlD phenotype.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Biological Transport, Active Chromosome Mapping Cloning, Molecular DNA, Bacterial/genetics Escherichia coli/genetics Genes, Bacterial Membrane Proteins/physiology Molybdenum/physiology Nitrate Reductase Nitrate Reductases/genetics
Chemicals
Bacterial Proteins DNA, Bacterial Membrane Proteins Molybdenum Nitrate Reductases Nitrate Reductase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Johann S
Hinton S M
References (28)
28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.5.1911-1916.1987
Published
1987-05-00
Pages
1911-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212044
Subset
IM
Databases
GENBANK
M16182
External Links
PubMed source DOI: 10.1128/jb.169.5.1911-1916.1987 PMC Full Text View journal details
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