Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.

Nature ·Vol. 327 ·No. 6117 ·1987-00-00 ·Pages 31-5

Lee MG, Nurse P

Abstract

A human homologue of the cdc2 gene has been cloned by expressing a human cDNA library in fission yeast and selecting for clones that can complement a mutant of cdc2. The predicted protein sequence of the human homologue is very similar to that of the yeast cdc2 gene. These data indicate that elements of the mechanism by which the cell cycle is controlled are likely to be conserved between yeast and humans.

MeSH Terms

Amino Acid Sequence Base Sequence CDC2 Protein Kinase Cell Cycle Cloning, Molecular DNA/genetics Fungal Proteins/genetics Genes Genes, Fungal Genetic Complementation Test Humans Phosphoproteins/genetics,physiology Protein Kinases/genetics,physiology Recombinant Proteins/genetics Saccharomycetales/genetics Schizosaccharomyces/genetics Sequence Homology, Nucleic Acid

Chemicals

Fungal Proteins Phosphoproteins Recombinant Proteins DNA Protein Kinases CDC2 Protein Kinase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Lee M G

Nurse P

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/327031a0

Published

1987-00-00

Pages

31-5

Language

English

Region

England

NLM ID

0410462

Subset

Databases

GENBANK

X05360, Y00272

External Links

PubMed source DOI: 10.1038/327031a0 View journal details

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Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.

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