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PMID: 3558253 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new diploid nontumorigenic human breast epithelial cell line isolated and propagated in chemically defined medium.

In vitro cellular & developmental biology : journal of the Tissue Culture Association ·Vol. 23 ·No. 3 ·1987-03-00 ·Pages 181-8

Briand P, Petersen OW, Van Deurs B

Abstract

A new, nontumorigenic human breast epithelial cell line, HMT-3522, has been established from fibrocystic breast tissue. Cells were explanted and propagated in chemically defined medium including insulin, transferrin, epidermal growth factor, hydrocortisone, estradiol, prolactin, and Na-selenite. The epithelial nature of the cell line was established by immunocytochemical detection of cytokeratins. Moreover, electronmicroscopy revealed monolayers of polarized cells connected by desmosomes and provided with apical microvilli. Milk fat globule membrane antigen, specific for the apical membrane domain of normal, luminal breast epithelial cells, was expressed only in confluent cultures where some cells overlaid others, indicating "stem cell"-like properties. After 25 to 30 passages, the cells are diploid with a few marker chromosomes and loss of chromosomes in the D-group. The cells are nontumorigenic in athymic mice; they lack estrogen receptors, and estradiol does not stimulate growth. The HMT-3522 cell line may represent a useful model for the study of breast cell differentiation and carcinogenesis in vitro.

MeSH Terms
Animals Antigens/analysis Breast/analysis,immunology,ultrastructure Cell Division Cell Line Culture Media Epithelium Female Fibrocystic Breast Disease/pathology Humans Membrane Proteins/analysis Mice Middle Aged Mucin-1 Receptors, Estrogen/analysis
Chemicals
Antigens Culture Media Membrane Proteins Mucin-1 Receptors, Estrogen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Briand P
Petersen O W
Van Deurs B
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49 references, click to expand
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Article Info
Journal
In vitro cellular & developmental biology : journal of the Tissue Culture Association
Abbr.
In Vitro Cell Dev Biol
ISSN
0883-8364
DOI
10.1007/BF02623578
Published
1987-03-00
Pages
181-8
Language
English
Region
United States
NLM ID
8506951
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02623578
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