Restriction enzyme cleavage mapping of T7 virus early region.

Molecular & general genetics : MGG ·Vol. 162 ·No. 3 ·1978-07-04 ·Pages 329-39

Gordon RL, Humphries P, McConnell DJ

Abstract

DNA molecules of seven T7 mutants with overlapping deletions in the early region were cleaved by restriction enzymes HindII, HpaI and II, and HaeIII. The differences in the cleavage patterns after electrophoresis have been used to generate a cleavage map of the restriction sites of this enzyme. It covers the first 9% of the T7 DNA molecule. Cleavage points for HindII are at 0.60, 1.33, 1.59, 1.76, 5.26, 6.27, 7.4 and 8.38%; for HpaI and II at 1.36, 1.62, 4.46, 6.29, 6.62, 7.56, and 8.76%, for HaeIII at 3.85, 6.98, 7.88 and 8.26%. Some fragments have been located in the region containing the early promoters, others carry the complete sequences of gene 0.3.

MeSH Terms

Coliphages/genetics DNA Restriction Enzymes/metabolism DNA, Viral/genetics Escherichia coli Genes, Viral Genetic Linkage Haemophilus/enzymology

Chemicals

DNA, Viral DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gordon R L

Humphries P

McConnell D J

References (28)

28 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00268859

Published

1978-07-04

Pages

329-39

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00268859

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Restriction enzyme cleavage mapping of T7 virus early region.

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