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PMID: 3565757 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Detection and characterization of lipid hydroperoxides at picomole levels by high-performance liquid chromatography.

Analytical biochemistry ·Vol. 160 ·No. 1 ·1987-01-00 ·Pages 7-13

Yamamoto Y, Brodsky MH, Baker JC, Ames BN

Abstract

A new method for the detection of various lipid hydroperoxides and hydrogen peroxide at the picomole level has been developed by combining an HPLC system with an ultrasensitive analytical system based on the detection of chemiluminescence emitted by isoluminol in the presence of hydroperoxide and microperoxidase. This HPLC separation removes interfering antioxidants so that the method can be applied to biological samples such as blood plasma lipids. Several HPLC conditions are described which allow simple identification of different lipid hydroperoxides.

MeSH Terms

Adult Antioxidants/analysis Chromatography, High Pressure Liquid/methods Humans Hydrogen Peroxide/analysis Lipid Peroxides/analysis,blood Luminescent Measurements Male Microchemistry Peroxidases

Chemicals

Antioxidants Lipid Peroxides Hydrogen Peroxide Peroxidases microperoxidase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Yamamoto Y

Brodsky M H

Baker J C

Ames B N

Article Info

Journal

Analytical biochemistry

Abbr.

Anal Biochem

ISSN

0003-2697

DOI

10.1016/0003-2697(87)90606-3

Published

1987-01-00

Pages

7-13

Language

English

Region

United States

NLM ID

0370535

Subset

Grants

NCI NIH HHS · CA39910 · United States

NIEHS NIH HHS · ES01896 · United States

NIGMS NIH HHS · GM19993 · United States

External Links

PubMed source DOI: 10.1016/0003-2697(87)90606-3 View journal details

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Detection and characterization of lipid hydroperoxides at picomole levels by high-performance liquid chromatography.

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