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PMID: 3580381 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Arachidonic acid epoxygenase: detection of epoxyeicosatrienoic acids in human urine.

Biochimica et biophysica acta ·Vol. 919 ·No. 2 ·1987-06-02 ·Pages 132-9

Toto R, Siddhanta A, Manna S, Pramanik B, Falck JR, Capdevila J

Abstract

Epoxyeicosatrienoic acids, metabolites of the cytochrome P-450-mediated epoxygenase reaction, were detected in human urine by gas chromatographic-mass spectroscopic techniques after conversion to their hydrogenated and non-hydrogenated methyl and pentafluorobenzyl esters. Initial analysis of the regioisomeric composition utilizing the corresponding hydrogenated pentafluorobenzyl esters revealed the presence of the 8,9- and 14,15-isomers.

MeSH Terms

8,11,14-Eicosatrienoic Acid/analogs & derivatives,urine Cytochrome P-450 CYP2J2 Cytochrome P-450 Enzyme System Fatty Acids, Unsaturated/urine Female Gas Chromatography-Mass Spectrometry Humans Isomerism Oxygenases/metabolism

Chemicals

Fatty Acids, Unsaturated 5,6-epoxy-8,11,14-eicosatrienoic acid Cytochrome P-450 Enzyme System Oxygenases Cytochrome P-450 CYP2J2 8,11,14-Eicosatrienoic Acid

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Toto R

Siddhanta A

Manna S

Pramanik B

Falck J R

Capdevila J

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/0005-2760(87)90199-8

Published

1987-06-02

Pages

132-9

Language

English

Region

Netherlands

NLM ID

0217513

Subset

Grants

ADAMHA HHS · NIADDKD-38226 · United States

NIADDK NIH HHS · R23 AM36319 01A1 · United States

External Links

PubMed source DOI: 10.1016/0005-2760(87)90199-8

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Arachidonic acid epoxygenase: detection of epoxyeicosatrienoic acids in human urine.

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