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PMID: 358146 Published · ppublish English Journal Article

Evidence for RNA-RNA cross-link formation in Escherichia coli ribosomes.

Nucleic acids research ·Vol. 5 ·No. 8 ·1978-08-00 ·Pages 2705-20

Zwieb C, Ross A, Rinke J, Meinke M, Brimacombe R

Abstract

Evidence is presented in three separate cases for the formation of RNA-RNA cross-links in intact E. coli ribosomes and ribosomal subunits. The first case is a cross-link between the 18S and 13S regions of the 23S RNA, induced by ultraviolet irradiation. The second is a cross-link at the subunit interface, generated by the bifunctional reagent bis-(2-chloroethyl)-amine. The third example is a cross-link between sections O'-D and P-A of the 16S RNA, induced as in the first case by ultraviolet irradiation. The RNA-RNA cross-links can be identified as such, despite the complications introduced by concomitant RNA-protein cross-linking reactions. The experiments represent a first attempt to introduce RNA-RNA cross-linking into studies of the topographical organization of the RNA within the ribosome.

MeSH Terms
Escherichia coli/ultrastructure Mechlorethamine Molecular Weight RNA, Ribosomal/radiation effects Ribosomes/ultrastructure Ultraviolet Rays
Chemicals
RNA, Ribosomal Mechlorethamine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zwieb C
Ross A
Rinke J
Meinke M
Brimacombe R
References (22)
22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.8.2705
Published
1978-08-00
Pages
2705-20
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342201
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.8.2705 PMC Full Text View journal details
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