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PMID: 358196 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Lysyl-derived aldehydes in outer membrane proteins of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 8 ·1978-08-00 ·Pages 3708-12

Diedrich DL, Schnaitman CA

Abstract

The major outer membrane proteins from Escherichia coli K-12 are modified to contain alpha-aminoadipic acid delta-semialdehyde (allysine). The allysine was found to be derived from lysine and it was identified by derivatizing it to chloronorleucine by reduction, alpha-aminoadipic acid by oxidation, and to alpha,epsilon-diaminopimelic acid by reacting it with CN- and NH3. The alpha-aminoadipic acid was identified by mass spectrometry. Two major outer membrane proteins were found to possess allysine, a modified lysine characteristically found to connective tissue.

MeSH Terms
2-Aminoadipic Acid/analogs & derivatives,analysis Aldehydes/analysis Amino Acids, Dicarboxylic/analysis Chromatography, Thin Layer Escherichia coli/metabolism Mass Spectrometry Membrane Proteins
Chemicals
Aldehydes Amino Acids, Dicarboxylic Membrane Proteins 2-Aminoadipic Acid allysine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Diedrich D L
Schnaitman C A
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.8.3708
Published
1978-08-00
Pages
3708-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392855
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.8.3708 PMC Full Text View journal details
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