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PMID: 3582365 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A novel endoribonuclease cleaves at a priming site of mouse mitochondrial DNA replication.

The EMBO journal ·Vol. 6 ·No. 2 ·1987-02-00 ·Pages 409-17

Chang DD, Clayton DA

Abstract

Priming at the mouse mitochondrial origin of heavy-strand DNA replication is effected by transcripts from the light-strand promoter. The transition from RNA synthesis to DNA synthesis occurs at specific locations between 75 and 165 nucleotides downstream from the transcriptional initiation site. We have identified and partially purified an endonucleolytic activity that cleaves RNA accurately near one of these transition sites; this finding implies a role of specific RNA processing in DNA replication. Cleavage products possess 5'-phosphoryl and 3'-hydroxyl termini. Heterologous assays using mouse or human mitochondrial endoribonuclease with substrates containing the sequences of the human or mouse mitochondrial origins of heavy-strand DNA replication suggest that selection of the cleavage site is guided by sequences adjacent to the actual position of cleavage.

MeSH Terms
Animals Base Sequence Cells, Cultured DNA Replication DNA, Mitochondrial/genetics,metabolism Endodeoxyribonucleases/isolation & purification,metabolism Mice Plasmids Substrate Specificity
Chemicals
DNA, Mitochondrial Endodeoxyribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chang D D
Clayton D A
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33 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-02-00
Pages
409-17
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553411
Subset
IM
Grants
NIGMS NIH HHS · GM-07365-11 · United States
NIGMS NIH HHS · GM-33088-16 · United States
External Links
PubMed source PMC Full Text View journal details
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