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PMID: 3601666 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Characterization of cDNA sequences corresponding to three distinct HMG-1 mRNA species in line CHO Chinese hamster cells and cell cycle expression of the HMG-1 gene.

Nucleic acids research ·Vol. 15 ·No. 13 ·1987-07-10 ·Pages 5051-68

Lee KL, Pentecost BT, D'Anna JA, Tobey RA, Gurley LR, Dixon GH

Abstract

We have isolated cDNA clones encoding the high mobility group (HMG) protein HMG-1 in line CHO Chinese hamster cells. The cDNA clones correspond to the three HMG-1 mRNA species detected on Northern blots. Three different polyadenylation sites are found to be used. The three mRNA species of sizes 1.05, 1.45 and 2.45 kb are generated by differential polyadenylation at sites 115 nucleotides, 513 nucleotides and 1515 nucleotides downstream from the stop codon. A perfectly conserved putative poly(A) signal AAUAAA is present upstream of only one of the three poly(A) sites. Two homologous but imperfect sequences exist upstream from the other two poly(A) sites. All three HMG-1 mRNA species maintain significant levels throughout the M, G1 and S phases of the cell cycle and the rate of large HMG protein (HMG-1 and HMG-2) synthesis increases approximately two-fold from G1 to S phase.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Cycle Cell Line Cloning, Molecular Cricetinae Cricetulus DNA/analysis Female Gene Expression Regulation Genes High Mobility Group Proteins/genetics Ovary RNA, Messenger/genetics
Chemicals
High Mobility Group Proteins RNA, Messenger DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lee K L
Pentecost B T
D'Anna J A
Tobey R A
Gurley L R
Dixon G H
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.13.5051
Published
1987-07-10
Pages
5051-68
Language
English
Region
England
NLM ID
0411011
PMCID
PMC305947
Subset
IM
Databases
GENBANK
Y00365
External Links
PubMed source DOI: 10.1093/nar/15.13.5051 PMC Full Text View journal details
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