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PMID: 3608983 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Biogenesis of the glycosome in Trypanosoma brucei: the synthesis, translocation and turnover of glycosomal polypeptides.

The EMBO journal ·Vol. 6 ·No. 5 ·1987-05-00 ·Pages 1403-11

Hart DT, Baudhuin P, Opperdoes FR, de Duve C

Abstract

Glycosomes, the microbodies of Trypanosoma brucei, contain a number of enzymes involved in glucose and glycerol metabolism. The biogenesis of three of these enzymes has been studied. Aldolase, D-glyceraldehyde-3-phosphate dehydrogenase and NAD-linked glycerol-3-phosphate dehydrogenase are all synthesized in the cytosol on free rather than on membrane-bound polysomes. In vitro, as well as in vivo, these polypeptides are synthesized at their mature size, and no evidence was found for any processing upon entry into the glycosomes. Continuous and pulse-chase labelling experiments with procyclic trypomastigotes revealed that the enzymes have a half-life in the cytosol of approximately 3 min or less, and then turn over rapidly in the glycosomes, with half-lives as short as 30 min.

MeSH Terms
Animals Fructose-Bisphosphate Aldolase/biosynthesis Glucosephosphate Dehydrogenase/biosynthesis Glyceraldehyde-3-Phosphate Dehydrogenases/biosynthesis Kinetics Microbodies/metabolism Protein Biosynthesis Proteins/genetics RNA, Messenger/genetics Trypanosoma brucei brucei/metabolism Tubulin/biosynthesis
Chemicals
Proteins RNA, Messenger Tubulin Glucosephosphate Dehydrogenase Glyceraldehyde-3-Phosphate Dehydrogenases Fructose-Bisphosphate Aldolase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hart D T
Baudhuin P
Opperdoes F R
de Duve C
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35 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-05-00
Pages
1403-11
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553946
Subset
IM
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