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PMID: 3609172 Published · ppublish English Journal Article

Characterization of non-typable strains of Staphylococcus aureus from cases of hospital infection.

Epidemiology and infection ·Vol. 99 ·No. 1 ·1987-08-00 ·Pages 191-200

Vindel A, Martín-Bourgon C, Saez-Nieto JA

Abstract

A high percentage of non-typable (NT) Staphylococcus aureus strains was isolated in Spanish hospitals during 1984 and 1985. Several alternative methods of typing were employed to study these isolates. These were: phage-typing at 1000 X RTD, phage-typing after heat-treatment (48 degrees C), thermal shock (56 degrees C), reverse-typing and induction of additional phages. Using these methods the number of NT isolates was reduced by 60%. Best results were obtained with heat-treatment. Additional phages and reverse-typing were also useful. A scheme for the study of outbreaks and sporadic cases caused by NT strains is proposed using the methods described.

MeSH Terms
Bacteriophage Typing Cross Infection/epidemiology,microbiology Disease Outbreaks Hot Temperature Humans Staphylococcal Infections/epidemiology Staphylococcus aureus/classification
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vindel A
Martín-Bourgon C
Saez-Nieto J A
References (9)
9 references, click to expand
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Article Info
Journal
Epidemiology and infection
Abbr.
Epidemiol Infect
ISSN
0950-2688
Published
1987-08-00
Pages
191-200
Language
English
Region
England
NLM ID
8703737
PMCID
PMC2249189
Subset
IM
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