Pyridine nucleotide-linked oxidation of methanol in methanol-assimilating yeasts.

Journal of bacteriology ·Vol. 124 ·No. 3 ·1975-12-00 ·Pages 1165-7

Mehta RJ

Abstract

An alcohol dehydrogenase linked to nicotinamide adenine dinucleotide and requiring glutathione has been isolated and partially purified from two methanol-assimilating yeasts. It differs from previously described methanol-oxidizing enzymes in pH optima, electron acceptor specificity, substrate specificity, inhibition pattern, and stability.

MeSH Terms

Alcohol Oxidoreductases/isolation & purification,metabolism Ascomycota/metabolism Candida/enzymology,metabolism Cell-Free System Chloromercuribenzoates/pharmacology Drug Stability Glutathione/metabolism Hydrogen-Ion Concentration Iodoacetamide/pharmacology Metals/pharmacology Methanol/metabolism NAD/metabolism Oxidation-Reduction Pichia/enzymology,metabolism Species Specificity

Chemicals

Chloromercuribenzoates Metals NAD Alcohol Oxidoreductases Glutathione Methanol Iodoacetamide

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Mehta R J

References (5)

5 references, click to expand

Demonstration of methanol dehydrogenase in methanol assimilating yeasts.
Experientia. 1975 Apr 15;31(4):407-8 PMID: 164367
Microbial growth on C-1 compounds. 6. Oxidation of methanol, formaldehyde and formate by methanol-grown Pseudomonas AM-1.
Biochem J. 1964 Nov;93(2):281-90 PMID: 4284499
Microbial assimilation of methanol. The ethanol- and methanol-oxidizing enzymes of the yeast Candida boidinii.
Eur J Biochem. 1973 Jul 2;36(1):250-6 PMID: 4354620
Studies on methanol-oxidizing bacteria. II. Purification and properties of methanol dehydrogenase from Pseudomonas RJ 1 .
Antonie Van Leeuwenhoek. 1973;39(2):303-12 PMID: 4541604
The microbial oxidation of methanol. The alcohol dehydrogenase of Pseudomonas sp. M27.
Biochem J. 1965 Sep;96(3):808-12 PMID: 5862419

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.124.3.1165-1167.1975

Published

1975-12-00

Pages

1165-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC236023

Subset

External Links

PubMed source DOI: 10.1128/jb.124.3.1165-1167.1975 PMC Full Text View journal details

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Pyridine nucleotide-linked oxidation of methanol in methanol-assimilating yeasts.

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