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PMID: 361698 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloned DNA fragment specifying major outer membrane protein a in Escherichia coli K-12.

Journal of bacteriology ·Vol. 136 ·No. 1 ·1978-10-00 ·Pages 369-80

Gayda RC, Markovitz A

Abstract

Plasmid pMC44 is a recombinant plasmid that contains a 2-megadalton EcoRI fragment of Escherichia coli K-12 DNA joined to the cloning vehicle, pSC101. The polypeptides specified by plasmid pMC44 were identified and compared with those specified by pSC101 to determine those that are unique to pMC44. Three polypeptides specified by plasmid pMC44 were localized in the cell envelope fraction of minicells: a Sarkosyl-insoluble outer membrane polypeptide (designated M2), specified by the cloned 2-megadalton DNA fragment, and two Sarkosyl-soluble membrane polypeptides specified by the cloning plasmid pSC101. Bacteria containing plasmid pMC44 synthesized quantities of M2 approximately equal to the most abundant E. coli K-12 outer membrane protein. Evidence is presented that outer membrane polypeptide M2, specified by the recombinant plasmid pMC44, is the normal E. coli outer membrane protein designated protein a by Lugtenberg and 3b by Schnaitman.

MeSH Terms
Bacterial Proteins/analysis,biosynthesis DNA, Bacterial/genetics DNA, Recombinant Escherichia coli/genetics,metabolism Membrane Proteins/analysis,biosynthesis Plasmids RNA, Bacterial/genetics RNA, Messenger/genetics
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Recombinant Membrane Proteins RNA, Bacterial RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gayda R C
Markovitz A
References (31)
31 references, click to expand
  1. Escherichia coli K-12 tolF mutants: alterations in protein composition of the outer membrane.
    J Bacteriol. 1977 May;130(2):781-6 PMID: 400794
  2. Altered bacteriophage lambda expression in cell division mutants capR(lon) of Escherichia coli K-12.
    Mol Gen Genet. 1978 Feb 7;159(1):1-11 PMID: 416333
  3. Protein expression in E. coli minicells by recombinant plasmids.
    Cell. 1977 Mar;10(3):521-36 PMID: 403011
  4. On the nature of tetracycline resistance controlled by the plasmid pSC101.
    Cell. 1978 Jan;13(1):73-81 PMID: 340050
  5. Outer membrane proteins of Escherichia coli. VI. Protein alteration in bacteriophage-resistant mutants.
    J Bacteriol. 1977 Aug;131(2):608-22 PMID: 328489
  6. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  7. New mini-ColE1 as a molecular cloning vehicle.
    J Bacteriol. 1977 Jan;129(1):358-66 PMID: 318644
  8. Role of a sugar-lipid intermediate in colanic acid synthesis by Escherichia coli.
    J Bacteriol. 1977 Jan;129(1):225-36 PMID: 318640
  9. Influence of cultural conditions and mutations on the composition of the outer membrane proteins of Escherichia coli.
    Mol Gen Genet. 1976 Sep 23;147(3):251-62 PMID: 787762
  10. Proteins specified by herpes simplex virus. VI. Viral proteins in the plasma membrane.
    J Virol. 1972 Mar;9(3):431-9 PMID: 4111436
  11. Outer membrane proteins of Escherichia coli. IV. Differences in outer membrane proteins due to strain and cultural differences.
    J Bacteriol. 1974 May;118(2):454-64 PMID: 4597444
  12. Derepression of anthranilate synthase in purified minicells of Escherichia coli containing the Col-trp plasmid.
    J Bacteriol. 1973 Aug;115(2):615-22 PMID: 4579874
  13. Mode of insertion of lipopolysaccharide into the outer membrane of escherichia coli.
    J Bacteriol. 1976 Apr;126(1):467-77 PMID: 770432
  14. Outer membrane proteins of Escherichia coli. 3. Evidence that the major protein of Escherichia coli O111 outer membrane consists of four distinct polypeptide species.
    J Bacteriol. 1974 May;118(2):442-53 PMID: 4208134
  15. R factor proteins synthesized in Escherichia coli minicells: membrane-associated R factor proteins.
    J Bacteriol. 1974 Dec;120(3):1464-71 PMID: 4612024
  16. R factor proteins synthesized in Escherichia coli minicells: incorporation studies with different R factors and detection of deoxyribonucleic acid-binding proteins.
    J Bacteriol. 1974 Dec;120(3):1451-63 PMID: 4612023
  17. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate.
    J Bacteriol. 1973 Sep;115(3):717-22 PMID: 4580564
  18. Differential inhibitory effects of antibiotics on the biosynthesis of envelope proteins of Escherichia coli.
    J Mol Biol. 1973 Sep 15;79(2):373-89 PMID: 4586413
  19. Specific biosynthesis of an envelope protein of Escherichia coli.
    Nature. 1973 Apr 6;242(5397):405-7 PMID: 4573580
  20. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.
    J Biol Chem. 1972 Jun 25;247(12):3962-72 PMID: 4555955
  21. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  22. The major proteins of the Excherichia coli outer cell envelope membrane. Preparative isolation of all major membrane proteins.
    Eur J Biochem. 1975 Nov 1;59(1):207-13 PMID: 812694
  23. Second-site mutations in capR (lon) strains of Escherichia coli K-12 that prevent radiation sensitivity and allow bacteriophage lambda to lysogenize.
    J Bacteriol. 1976 Sep;127(3):1208-16 PMID: 783136
  24. Outer membrane of Escherichia coli K-12: differentiation of proteins 3A and 3B on acrylamide gels and further characterization of con (tolG) mutants.
    J Bacteriol. 1976 Sep;127(3):1070-9 PMID: 783128
  25. Very stable prokaryotic messenger RNA in chromosomeless Escherichia coli minicells.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):2900-4 PMID: 1103125
  26. Production, properties and utility of bacterial minicells.
    Curr Top Microbiol Immunol. 1975;69:1-84 PMID: 1098854
  27. Synthesis of an R plasmid protein associated with tetracycline resistance is negatively regulated.
    Proc Natl Acad Sci U S A. 1976 May;73(5):1509-12 PMID: 775491
  28. Cloning of Escherichia coli DNA that controls cell division and capsular polysaccharide synthesis.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):697-701 PMID: 768981
  29. The major proteins of the Escherichia coli outer cell-envelope membrane. Heterogeneity of protein I.
    Eur J Biochem. 1976 Mar 16;63(1):47-52 PMID: 770169
  30. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands.
    FEBS Lett. 1975 Oct 15;58(1):254-8 PMID: 773686
  31. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.136.1.369-380.1978
Published
1978-10-00
Pages
369-80
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218669
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.136.1.369-380.1978 PMC Full Text View journal details
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