A DNA segment within the colicin E1 structural gene on ColE1 affecting immunity to colicin.

Molecular & general genetics : MGG ·Vol. 164 ·No. 3 ·1978-09-08 ·Pages 259-64

Shafferman A, Cohen S, Flashner Y

Abstract

Insertion of DNA at the EcoRI site of ColE1 results in increase of immunity to colicin killing in E. coli harboring such recombinant ColE1 plasmid as compared to E. coli (ColE1). This effect is neither due to cis or trans interactions originating from the inserted foreign DNA fragment, nor to changes in plasmid copy number. This defect in the immunity mechanism is not trans complemented for by wild type ColE1. Increase in immunity can also be obtained by deleting a DNA segment from the ColE1 genome. This segment is approximately 120 bp left to the EcoRI site within the colicin structural gene. It is concluded that the structure of DNA per se, around the EcoRI site, within colcin structural gene, is the structure which affects immunity expression.

MeSH Terms

Colicins/immunology DNA, Bacterial/genetics Escherichia coli/genetics Genes Plasmids Recombination, Genetic Transcription, Genetic Transformation, Bacterial

Chemicals

Colicins DNA, Bacterial

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Shafferman A

Cohen S

Flashner Y

References (16)

16 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00333155

Published

1978-09-08

Pages

259-64

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00333155

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A DNA segment within the colicin E1 structural gene on ColE1 affecting immunity to colicin.

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