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PMID: 363683 Published · ppublish English Journal Article

Chromosomal superkiller mutants of Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 136 ·No. 3 ·1978-12-00 ·Pages 1002-7

Toh-E A, Guerry P, Wickner RB

Abstract

Yeast strains carrying a 1.5 X 10(6)-dalton double-stranded RNA in virus-like particles secrete a protein toxin which is lethal to strains not carrying this species of double-stranded RNA. We find that recessive mutations in any of four chromosomal genes result in the superkiller phenotype, i.e., increased secretion of killer toxin activity by strains carrying the killer genome. These genes are designated ski1 through ski4 (for superkiller), ski3 and ski4 are located on chromosome XIV, and ski1 is on chromosome VII. A ski1 mutation results in a decreased rate of cell growth. The kex1 and kex2 mutations are epistatic to each ski mutation.

MeSH Terms
Chromosome Mapping Fungal Proteins/metabolism Genes, Recessive Genetic Linkage Mutation Mycotoxins/metabolism Plasmids RNA/analysis Saccharomyces cerevisiae/genetics,metabolism
Chemicals
Fungal Proteins Mycotoxins RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Toh-E A
Guerry P
Wickner R B
References (22)
22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.136.3.1002-1007.1978
Published
1978-12-00
Pages
1002-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218536
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.136.3.1002-1007.1978 PMC Full Text View journal details
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