Abstract
Streptococcus suis types 1 and 2 were subjected to digestion with lysozyme. Serologically type-specific capsular polysaccharides were isolated from the lysates by ethanol precipitation followed by Sepharose 6B chromatography. The purified type 1 polysaccharide has a Kd value of 0.074 on a Sepharose 4B column and contains galactose, glucose, N-acetyl glucosamine, N-acetyl galactosamine, and sialic acid in a molar ratio of 2.42:1.00:1.00:1.13:1.39. The type 2 polysaccharide has a Kd value of 0.185 and is composed of rhamnose, galactose, glucose, N-acetyl glucosamine, and sialic acid in a molar ratio of 1.07:3.17:1.00:0.94:1.00. A comparison is drawn between the type polysaccharides of S. suis and those of group B streptococci.
MeSH Terms
Carbohydrates/analysis
Polysaccharides, Bacterial/immunology,isolation & purification
Streptococcus/immunology
Streptococcus agalactiae/immunology
Chemicals
Carbohydrates
Polysaccharides, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Elliott S D
Tai J Y
References (12)
12 references, click to expand
-
TYPING GROUP A HEMOLYTIC STREPTOCOCCI BY M PRECIPITIN REACTIONS IN CAPILLARY PIPETTES.
J Exp Med. 1943 Aug 1;78(2):127-33
PMID: 19871314
-
Teichoic acids of group D streptococci with special reference to strains from pig meningitis (Streptococcus suis).
J Exp Med. 1977 Mar 1;145(3):490-9
PMID: 45589
-
Immunochemical characterization of the "native" type III polysaccharide of group B Streptococcus.
J Exp Med. 1976 Feb 1;143(2):258-70
PMID: 55450
-
Existence of multiple immunodeterminants in the type-specific capsular substance of group B type Ia streptococci.
Infect Immun. 1978 Mar;19(3):983-91
PMID: 76609
-
Complete amino acid analysis of proteins from a single hydrolysate.
J Biol Chem. 1976 Apr 10;251(7):1936-40
PMID: 178649
-
MOLECULAR EXCLUSION AND RESTRICTED DIFFUSION PROCESSES IN MOLECULAR-SIEVE CHROMATOGRAPHY.
Biochemistry. 1964 May;3:723-30
PMID: 14193644
-
Streptococcal infection in young pigs. IV. An outbreak of streptococcal meningitis in weaned pigs.
J Hyg (Lond). 1975 Aug;75(1):69-78
PMID: 807620
-
Multiple mouse-protective antibodies directed against group B streptococci. Special reference to antibodies effective against protein antigens.
J Exp Med. 1975 Jul 1;142(1):165-79
PMID: 1097573
-
Streptococcal infection in young pigs. I. An immunochemical study of the causative agent (PM streptococcus).
J Hyg (Lond). 1966 Jun;64(2):205-12
PMID: 5220563
-
Type and group polysaccharides of group D streptococci.
J Exp Med. 1960 May 1;111:621-30
PMID: 13726456
-
SEPTICAEMIC INFECTIONS IN PIGS, CAUSED BY HAEMOLYTIC STREPTOCOCCI OF NEW LANCEFIELD GROUPS DESIGNATED R, S, AND T.
Antonie Van Leeuwenhoek. 1963;29:272-80
PMID: 14068459
-
Meningitis and endophthalmitis caused by Streptococcus suis type II (group R).
Br Med J. 1977 Jul 16;2(6080):167-8
PMID: 301419