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PMID: 36398248 Published · epublish English Journal Article

lncRNA EPS15-AS1 affects the biological behavior of liver cancer stem cells by regulating EPS15 expression.

American journal of translational research ·Vol. 14 ·No. 10 ·2022-00-00 ·Pages 7324-7335

Liu G, Lan Y, Yin X, Wu T

Abstract

To investigate whether long non-coding RNA (lncRNA) EPS15-antisense RNA (EPS15-AS1) affects the biological behavior of liver cancer stem cells (LCSCs) by targeting EPS15. The expression of EPS15 in liver cancer was analyzed based on TCGA database. The expression of EPS15-AS1 and EPS15 in LCSCs was detected by real-time quantitative PCR (RT-qPCR). MTT method, flow cytometry, and Transwell assay were used to detect the effects of EPS15-AS1 and EPS15 expression on the biological behavior of LCSCs. EPS15 was highly expressed in liver cancer tissues in TCGA, and EPS15 was closely related to the survival and prognosis of liver cancer patients (P<0.05). EPS15 was highly expressed in LCSCs, while lncRNA EPS15-AS1 was lowly expressed in LCSCs (P<0.05). After silencing lncRNA EPS15-AS1, the proliferation, invasion, and EPS15 protein expression of LCSCs were promoted (P<0.05) while apoptosis was suppressed (P<0.05). After overexpression of lncRNA EPS15-AS1, the proliferation, invasion, and EPS15 protein expression of LCSCs were suppressed while the apoptosis ability was promoted. However, simultaneous overexpression of lncRNA EPS15-AS1 and EPS15 attenuated the effect of lncRNA EPS15-AS1 overexpression alone on proliferation and apoptosis of LCSCs. lncRNA EPS15-AS1 overexpression can inhibit proliferation and invasion but promote apoptosis of LCSCs by down-regulating the expression of EPS15.

Keywords
EPS15 apoptosis invasion liver cancer stem cells proliferation
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Liu Guodong
Hepatobiliary Pancreatic Surgery, Hefei Cancer Hospital, Chinese Academy of Sciences Hefei 230031, Anhui Province, P. R. China.
Lan Yadong
Hepatobiliary Pancreatic Surgery, Hefei Cancer Hospital, Chinese Academy of Sciences Hefei 230031, Anhui Province, P. R. China.
Yin Xiaoqiang
Hepatobiliary Pancreatic Surgery, Hefei Cancer Hospital, Chinese Academy of Sciences Hefei 230031, Anhui Province, P. R. China.
Wu Tongchui
Hepatobiliary Pancreatic Surgery, Hefei Cancer Hospital, Chinese Academy of Sciences Hefei 230031, Anhui Province, P. R. China.
Conflict of Interest

None.

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Article Info
Journal
American journal of translational research
Abbr.
Am J Transl Res
ISSN
1943-8141
Published
2022-00-00
Epub
2022-00-15
Pages
7324-7335
Language
English
Region
United States
NLM ID
101493030
PMCID
PMC9641472
External Links
PubMed source PMC Full Text View journal details
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