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PMID: 365108 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Primary Klebsiella identification with MacConkey-inositol-carbenicillin agar.

Applied and environmental microbiology ·Vol. 36 ·No. 3 ·1978-09-00 ·Pages 536-8

Bagley ST, Seidler RJ

Abstract

MacConkey-inositol-carbenicillin agar has successfully been used as a primary selective medium for Klebsiella enumeration. With pure cultures, nearly 100% recovery of Klebsiella was observed by membrane filtration. With environmental samples using membrane filtration, 95% of typical pink- to red-colored colonies were verified as Klebsiella, as opposed to only 1% of yellow background colonies. Recovery of Klebsiella on MacConkey-inositol-carbenicillin agar was as good or better than on mEndo agar LES (Difco Laboratories). Recovery and percent colony confirmation with MacConkey-inositol-carbenicillin agar were greater than for other proposed Klebsiella selective media.

MeSH Terms

Agar Carbenicillin Fresh Water Humans Inositol Klebsiella/isolation & purification Klebsiella Infections/microbiology Klebsiella pneumoniae/isolation & purification Sewage Species Specificity Water Microbiology

Chemicals

Sewage Inositol Agar Carbenicillin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Bagley S T

Seidler R J

References (8)

8 references, click to expand

Significance of fecal coliform-positive Klebsiella.
Appl Environ Microbiol. 1977 May;33(5):1141-8 PMID: 18086
Klebsielleae in drinking water emanating from redwood tanks.
Appl Environ Microbiol. 1977 Apr;33(4):893-900 PMID: 326189
Significance of low-temperature growth associated with the fecal coliform response, indole production, and pectin liquefaction in Klebsiella.
Appl Environ Microbiol. 1978 Feb;35(2):392-6 PMID: 637539
Methyl violet: a selective agent for differentiation of Klebsiella pneumoniae from Enterobacter aerogenes and other gram-negative organisms.
Appl Microbiol. 1975 Aug;30(2):258-61 PMID: 809008
Evaluation of double violet agar in the isolation of Klebsiella pneumoniae from river water.
Appl Environ Microbiol. 1976 Feb;31(2):213-5 PMID: 793520
Prevalence and characteristics of Klebsiella species: relation to association with a hospital environment.
J Infect Dis. 1974 Oct;130(4):402-405 PMID: 4613760
Characterization of Klebsiella isolates from natural receiving waters and comparison with human isolates.
Appl Microbiol. 1974 Oct;28(4):672-8 PMID: 4607526
Inositol as a selective substrate for the growth of Klebsiellae and Serratiae.
Zentralbl Bakteriol Orig A. 1976 Aug;235(4):453-8 PMID: 790856

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.36.3.536-538.1978

Published

1978-09-00

Pages

536-8

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC243082

Subset

External Links

PubMed source DOI: 10.1128/aem.36.3.536-538.1978 PMC Full Text View journal details

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Primary Klebsiella identification with MacConkey-inositol-carbenicillin agar.

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