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PMID: 3658669 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group.

Nucleic acids research ·Vol. 15 ·No. 17 ·1987-09-11 ·Pages 6799-811

Cornelissen BJ, Horowitz J, van Kan JA, Goldberg RB, Bol JF

Abstract

Infection of Samsun NN tobacco with tobacco mosaic virus (TMV) was found to induce the synthesis of mRNA encoding a basic protein with a 67% amino acid sequence homology to the known acidic pathogenesis-related (PR) proteins 1a, 1b and 1c. By Southern blot hybridization it was shown that the tobacco genome contains at least eight genes for acidic PR-1 proteins and a similar number of genes encoding the basic homologues. Clones corresponding to three of the genes for acidic PR-1 proteins were isolated from a genomic library of Samsun NN tobacco. The nucleotide sequence of these genes and their flanking sequences were determined. One clone was found to correspond to the PR-1a gene; the two other clones do not correspond to known TMV-induced PR-1 mRNA's and may represent silent genes. Compared to the PR-1a gene, these genes contain an insertion or deletion in the putative promoter region and mutations affecting the PR-1 reading frame.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Genes Genes, Viral Molecular Sequence Data Nucleic Acid Hybridization Plants/genetics Plants, Toxic RNA, Messenger/genetics Tobacco/genetics Tobacco Mosaic Virus/genetics Transcription, Genetic Viral Proteins/genetics
Chemicals
RNA, Messenger Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cornelissen B J
Department of Biochemistry, Leiden University, The Netherlands.
Horowitz J
van Kan J A
Goldberg R B
Bol J F
References (17)
17 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. New versatile cloning and sequencing vectors based on bacteriophage M13.
    Gene. 1983 Dec;26(1):91-9 PMID: 6323254
  3. Alkaline transfer of DNA to plastic membrane.
    Biochem Biophys Res Commun. 1984 Jul 18;122(1):340-4 PMID: 6743336
  4. Purification and partial characterization of the major "pathogenesis-related" tomato leaf protein P14 from potato spindle tuber viroid (PSTV)-infected tomato leaves.
    Arch Virol. 1984;81(3-4):263-84 PMID: 6477130
  5. Lambda Charon vectors (Ch32, 33, 34 and 35) adapted for DNA cloning in recombination-deficient hosts.
    Gene. 1983 Dec;26(2-3):171-9 PMID: 6323258
  6. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  7. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  8. Amino acid sequence of the ;pathogenesis-related' leaf protein p14 from viroid-infected tomato reveals a new type of structurally unfamiliar proteins.
    EMBO J. 1985 Nov;4(11):2745-9 PMID: 16453639
  9. Molecular cloning and selection of genes regulated in Aspergillus development.
    Cell. 1980 Oct;21(3):709-15 PMID: 6449291
  10. A tobacco mosaic virus-induced tobacco protein is homologous to the sweet-tasting protein thaumatin.
    Nature. 1986 May 29-Jun 4;321(6069):531-2 PMID: 3713832
  11. Rapid DNA isolations for enzymatic and hybridization analysis.
    Methods Enzymol. 1980;65(1):404-11 PMID: 6246361
  12. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  13. Structure and flanking regions of soybean seed protein genes.
    Cell. 1982 Jun;29(2):651-60 PMID: 6288266
  14. cDNA cloning of six mRNAs induced by TMV infection of tobacco and a characterization of their translation products.
    EMBO J. 1986 Sep;5(9):2057-61 PMID: 16453701
  15. A synthetic heat-shock promoter element confers heat-inducibility on the herpes simplex virus thymidine kinase gene.
    EMBO J. 1982;1(11):1473-7 PMID: 6327271
  16. Molecular characterization of messenger RNAs for 'pathogenesis related' proteins la, lb and lc, induced by TMV infection of tobacco.
    EMBO J. 1986 Jan;5(1):37-40 PMID: 16453665
  17. Virus-induced synthesis of messenger RNAs for precursors of pathogenesis-related proteins in tobacco.
    EMBO J. 1985 Sep;4(9):2167-71 PMID: 15938049
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.17.6799
Published
1987-09-11
Pages
6799-811
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306176
Subset
IM
Databases
GENBANK
X05959
External Links
PubMed source DOI: 10.1093/nar/15.17.6799 PMC Full Text View journal details
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