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PMID: 3658672 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

alpha-DNA-V. Parallel annealing, handedness and conformation of the duplex of the unnatural alpha-hexadeoxyribonucleotide alpha-[d(CpApTpGpCpG)] with its beta-complement beta-[d(GpTpApCpGpC)] deduced from high field 1H-NMR.

Nucleic acids research ·Vol. 15 ·No. 17 ·1987-09-11 ·Pages 7027-44

Morvan F, Rayner B, Imbach JL, Lee M, Hartley JA, Chang DK, Lown JW

Abstract

The beta-complementary hexamer, beta-d[GTACGC], to the alpha-sequence, alpha-d[CATGCG], was synthesized by the phosphotriester method. The non-exchangeable proton assignments were obtained using 1D- and 2D-NMR techniques, including NOE, COSY and NOESY. The beta-strand exists as a random coil at 21 degrees C; however, at 4 degrees C, it forms an antiparallel self-recognition duplex annealing at positions 1-4. The beta-strand was annealed to the alpha-strand, and confirmation of complete annealing was obtained by detection and assignment of the six base pair imino protons in H2O/D2O solution at 21 degrees C. 1D-NOE experiments of the alpha, beta duplex d[alpha-(CATGCG) X beta-(GTACGC)] reveal that (i) it exists in aqueous solution in a conformation that belongs to the B family, (ii) it is 70 +/- 10% right-handed, (iii) the sugar-base orientations of the beta-strand are anti, and the deoxyribose units exist predominantly in the 2'-endo-3'-exo conformation. NOE measurements of the imino proton signals in the alpha, beta duplex reveal that the duplex exhibits parallel polarity.

MeSH Terms
Base Composition Base Sequence DNA Hydrogen Magnetic Resonance Spectroscopy/methods Nucleic Acid Conformation Oligodeoxyribonucleotides Thermodynamics
Chemicals
Oligodeoxyribonucleotides Hydrogen DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Morvan F
Université des Sciences et Techniques du Languedoc, UA 488 du CNRS, Montpellier, France.
Rayner B
Imbach J L
Lee M
Hartley J A
Chang D K
Lown J W
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.17.7027
Published
1987-09-11
Pages
7027-44
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306190
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.17.7027 PMC Full Text View journal details
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