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PMID: 3663127 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Kinetic properties of hexokinase as assembled with a microcomputer data base.

The Biochemical journal ·Vol. 244 ·No. 2 ·1987-06-01 ·Pages 351-7

Garfinkel L, Garfinkel D, Matsiras P, Matschinsky B

Abstract

We have constructed a relational data base containing the kinetic properties of the isoenzymes of hexokinase using the Knowledgeman data-base program on an IBM PC microcomputer. The natural subunit of this data base is the refereed publication, 165 of which were included. Reported values for the Mr (approx. 97,000) are in good agreement, but this agreement becomes progressively worse as one examines the Km values for glucose and ATP and the Ki for glucose 6-phosphate, where the reported values are spread over three orders of magnitude. Some quantities are very thinly or unreliably determined. Experimental conditions, especially free Mg2+ concentration, are rarely close to physiological. Reasons for the spread or uncertainty of numbers, and the distinctions that can be made between isoenzymes despite this spread, are discussed.

MeSH Terms
Adenosine Triphosphate/metabolism Computers Enzyme Activation/drug effects Glucose/metabolism Hexokinase/antagonists & inhibitors,metabolism Isoenzymes/metabolism Kinetics Mannose/metabolism Microcomputers Molecular Weight Substrate Specificity
Chemicals
Isoenzymes Adenosine Triphosphate Hexokinase Glucose Mannose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Garfinkel L
Department of Computer Science, University of Pennsylvania, Philadelphia 19104.
Garfinkel D
Matsiras P
Matschinsky B
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24 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2440351
Published
1987-06-01
Pages
351-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1147998
Subset
IM
Grants
NIADDK NIH HHS · AM 33016 · United States
External Links
PubMed source DOI: 10.1042/bj2440351 PMC Full Text View journal details
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