Porphyrin test as an alternative to benzidine test for detecting cytochromes in catalase-negative gram-positive cocci.

Journal of clinical microbiology ·Vol. 25 ·No. 10 ·1987-10-00 ·Pages 2006-7

Wong JD

Abstract

A total of 66 strains of gram-positive cocci, including 21 catalase-negative members of the family Streptococcaceae and strains of Stomatococcus mucilaginosus, were investigated for the ability to produce porphobilinogen and porphyrin from delta-aminolevulinic acid as an alternative to the benzidine test for detecting the presence of cytochromes. Production of porphobilinogen correlated 100% with membership in the family Micrococcaceae.

MeSH Terms

Aminolevulinic Acid/metabolism Catalase/biosynthesis Cytochromes/analysis Gram-Positive Bacteria/analysis,classification,metabolism Micrococcaceae/analysis,classification,metabolism Porphobilinogen/biosynthesis Porphyrins/biosynthesis Streptococcaceae/analysis,classification,metabolism

Chemicals

Cytochromes Porphyrins Porphobilinogen Aminolevulinic Acid Catalase

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Wong J D

Microbial Diseases Laboratory, California State Department of Health Services, Berkeley 94704.

References (5)

5 references, click to expand

Physiological differentiation of viridans streptococci.
J Clin Microbiol. 1977 Feb;5(2):184-201 PMID: 845245
Modified oxidase and benzidine tests for separation of staphylococci from micrococci.
J Clin Microbiol. 1981 Jun;13(6):1031-5 PMID: 7019234
The gram positive cocci.
Hum Pathol. 1976 Mar;7(2):187-94 PMID: 1262015
Simplified scheme for routine identification of human Staphylococcus species.
J Clin Microbiol. 1975 Jan;1(1):82-8 PMID: 170303
Modified benzidine test for the detection of cytochrome-containing respiratory systems in microorganisms.
J Bacteriol. 1960 Mar;79:356-60 PMID: 13815446

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.25.10.2006-2007.1987

Published

1987-10-00

Pages

2006-7

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC269388

Subset

External Links

PubMed source DOI: 10.1128/jcm.25.10.2006-2007.1987 PMC Full Text View journal details

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Porphyrin test as an alternative to benzidine test for detecting cytochromes in catalase-negative gram-positive cocci.

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