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PMID: 3671068 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Deduced products of C4-dicarboxylate transport regulatory genes of Rhizobium leguminosarum are homologous to nitrogen regulatory gene products.

Nucleic acids research ·Vol. 15 ·No. 19 ·1987-10-12 ·Pages 7921-34

Ronson CW, Astwood PM, Nixon BT, Ausubel FM

Abstract

We have sequenced two genes dctB and dctD required for the activation of the C4-dicarboxylate transport structural gene dctA in free-living Rhizobium leguminosarum. The hydropathic profile of the dctB gene product (DctB) suggested that its N-terminal region may be located in the periplasm and its C-terminal region in the cytoplasm. The C-terminal region of DctB was strongly conserved with similar regions of the products of several regulatory genes that may act as environmental sensors, including ntrB, envZ, virA, phoR, cpxA, and phoM. The N-terminal domains of the products of several regulatory genes thought to be transcriptional activators, including ntrC, ompR, virG, phoB and sfrA. In addition, the central and C-terminal regions of DctD were strongly conserved with the products of ntrC and nifA, transcriptional activators that require the alternate sigma factor rpoN (ntrA) as co-activator. The central region of DctD also contained a potential ATP-binding domain. These results are consistent with recent results that show that rpoN product is required for dctA activation, and suggest that DctB plus DctD-mediated transcriptional activation of dctA may be mechanistically similar to NtrB plus NtrC-mediated activation of glnA in E. coli.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Transport Dicarboxylic Acids/metabolism Genes, Bacterial Genes, Regulator Models, Biological Molecular Sequence Data Nitrogen Fixation/genetics Rhizobium/genetics,metabolism Sequence Homology, Nucleic Acid
Chemicals
Dicarboxylic Acids
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ronson C W
Grasslands Division, Department of Scientific and Industrial Research, Palmerston North, New Zealand.
Astwood P M
Nixon B T
Ausubel F M
References (57)
57 references, click to expand
  1. Nucleotide sequence and transcriptional initiation site of two Pseudomonas aeruginosa pilin genes.
    J Biol Chem. 1986 Nov 25;261(33):15703-8 PMID: 2430961
  2. Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins.
    EMBO J. 1986 Feb;5(2):441-7 PMID: 3011408
  3. Nucleotide sequence of the phoR gene, a regulatory gene for the phosphate regulon of Escherichia coli.
    J Mol Biol. 1986 Dec 5;192(3):549-56 PMID: 3550103
  4. Characterization of the spo0A locus and its deduced product.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2647-51 PMID: 3157992
  5. ATP-binding site of adenylate kinase: mechanistic implications of its homology with ras-encoded p21, F1-ATPase, and other nucleotide-binding proteins.
    Proc Natl Acad Sci U S A. 1986 Feb;83(4):907-11 PMID: 2869483
  6. Multiple forms of the CheB methylesterase in bacterial chemosensing.
    J Biol Chem. 1985 Aug 25;260(18):10161-8 PMID: 2991277
  7. Mutations specifically affecting ligand interaction of the Trg chemosensory transducer.
    J Bacteriol. 1986 Jul;167(1):101-9 PMID: 3087946
  8. DNA sequence analysis of the dye gene of Escherichia coli reveals amino acid homology between the dye and OmpR proteins.
    J Biol Chem. 1985 Apr 10;260(7):4236-42 PMID: 2984198
  9. Nucleotide sequence of the phoM region of Escherichia coli: four open reading frames may constitute an operon.
    J Bacteriol. 1986 Oct;168(1):294-302 PMID: 3531171
  10. Two-component regulatory systems responsive to environmental stimuli share strongly conserved domains with the nitrogen assimilation regulatory genes ntrB and ntrC.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7850-4 PMID: 3020561
  11. An artificial anchor domain: hydrophobicity suffices to stop transfer.
    Cell. 1985 Jun;41(2):607-14 PMID: 3886166
  12. Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli.
    J Bacteriol. 1986 Jan;165(1):161-6 PMID: 3510184
  13. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  14. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
    EMBO J. 1982;1(8):945-51 PMID: 6329717
  15. The nucleotide sequence of the nitrogen regulation gene ntrB and the glnA-ntrBC intergenic region of Klebsiella pneumoniae.
    Nucleic Acids Res. 1985 Nov 11;13(21):7591-606 PMID: 2999699
  16. Genetic and physiological tests of three phosphate-specific transport mutants of Escherichia coli.
    J Bacteriol. 1981 Mar;145(3):1249-56 PMID: 7009575
  17. Sensory transducers of E. coli are composed of discrete structural and functional domains.
    Cell. 1983 Jun;33(2):615-22 PMID: 6305515
  18. Nucleotide sequence surrounding transcription initiation site of xylABC operon on TOL plasmid of Pseudomonas putida.
    Proc Natl Acad Sci U S A. 1984 Mar;81(6):1688-91 PMID: 6324212
  19. Conserved domains in bacterial regulatory proteins that respond to environmental stimuli.
    Cell. 1987 Jun 5;49(5):579-81 PMID: 3555840
  20. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.
    Nucleic Acids Res. 1982 Aug 11;10(15):4731-51 PMID: 7133997
  21. The Cpx proteins of Escherichia coli K12. Immunologic detection of the chromosomal cpxA gene product.
    J Biol Chem. 1986 Apr 5;261(10):4698-705 PMID: 3007473
  22. Succinate transport in Rhizobium leguminosarum.
    J Bacteriol. 1981 Oct;148(1):193-202 PMID: 7287623
  23. Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins.
    J Bacteriol. 1985 Nov;164(2):578-84 PMID: 2997120
  24. Genetic analysis of the major outer membrane proteins of Escherichia coli.
    Annu Rev Genet. 1981;15:91-142 PMID: 7039497
  25. Promoter mapping and cell cycle regulation of flagellin gene transcription in Caulobacter crescentus.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1142-6 PMID: 3469658
  26. Chimeric chemosensory transducers of Escherichia coli.
    Proc Natl Acad Sci U S A. 1985 Mar;82(5):1326-30 PMID: 3883356
  27. Regulation of the pho regulon in Escherichia coli K-12. Genetic and physiological regulation of the positive regulatory gene phoB.
    J Mol Biol. 1983 Aug 15;168(3):477-88 PMID: 6310121
  28. Identification and characterization of the Rhizobium meliloti ntrC gene: R. meliloti has separate regulatory pathways for activation of nitrogen fixation genes in free-living and symbiotic cells.
    J Bacteriol. 1987 Apr;169(4):1423-32 PMID: 2881918
  29. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  30. Characterization of the virA locus of Agrobacterium tumefaciens: a transcriptional regulator and host range determinant.
    EMBO J. 1987 Apr;6(4):849-56 PMID: 3595559
  31. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  32. Nucleotide sequence of the phoB gene, the positive regulatory gene for the phosphate regulon of Escherichia coli K-12.
    J Mol Biol. 1986 Jul 5;190(1):37-44 PMID: 3537313
  33. Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame.
    Nucleic Acids Res. 1987 Jan 12;15(1):31-49 PMID: 3029674
  34. Nucleotide sequence of the virulence gene virG of the Agrobacterium tumefaciens octopine Ti plasmid: significant homology between virG and the regulatory genes ompR, phoB and dye of E. coli.
    Nucleic Acids Res. 1986 Dec 22;14(24):9933-42 PMID: 3027669
  35. Homologies between the Salmonella typhimurium CheY protein and proteins involved in the regulation of chemotaxis, membrane protein synthesis, and sporulation.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):7989-93 PMID: 2999789
  36. Transcription of glnA in E. coli is stimulated by activator bound to sites far from the promoter.
    Cell. 1986 Jun 20;45(6):785-92 PMID: 2871943
  37. Nitrogen fixation specific regulatory genes of Klebsiella pneumoniae and Rhizobium meliloti share homology with the general nitrogen regulatory gene ntrC of K. pneumoniae.
    Nucleic Acids Res. 1985 Jun 25;13(12):4539-55 PMID: 2989799
  38. Covalent modification of the glnG product, NRI, by the glnL product, NRII, regulates the transcription of the glnALG operon in Escherichia coli.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):5909-13 PMID: 2874557
  39. A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene.
    Nature. 1982 Nov 11;300(5888):149-52 PMID: 7133135
  40. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  41. Protein-DNA recognition.
    Annu Rev Biochem. 1984;53:293-321 PMID: 6236744
  42. Identification of the promoter of the Pseudomonas gene coding for carboxypeptidase G2.
    J Mol Appl Genet. 1985;3(1):26-35 PMID: 3839252
  43. Nucleotide sequence of the genes involved in phosphate transport and regulation of the phosphate regulon in Escherichia coli.
    J Mol Biol. 1985 Jul 20;184(2):241-50 PMID: 2993631
  44. Regulation of nitrogen fixation genes.
    Annu Rev Genet. 1986;20:567-91 PMID: 3545064
  45. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  46. Molecular cloning and genetic organization of C4-dicarboxylate transport genes from Rhizobium leguminosarum.
    J Bacteriol. 1984 Dec;160(3):903-9 PMID: 6094513
  47. Fine structure of a membrane anchor domain.
    J Mol Biol. 1985 Jan 5;181(1):111-21 PMID: 3872373
  48. Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions.
    J Bacteriol. 1987 Jun;169(6):2424-31 PMID: 3034856
  49. Mechanism of activation of a human oncogene.
    Nature. 1982 Nov 11;300(5888):143-9 PMID: 6290897
  50. A gene essential for Agrobacterium virulence is homologous to a family of positive regulatory loci.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8278-82 PMID: 3022288
  51. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  52. Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships.
    J Bacteriol. 1985 Jan;161(1):189-98 PMID: 3881386
  53. Establishing homologies in protein sequences.
    Methods Enzymol. 1983;91:524-45 PMID: 6855599
  54. Symbiotic properties of C4-dicarboxylic acid transport mutants of Rhizobium leguminosarum.
    J Bacteriol. 1983 Jun;154(3):1403-13 PMID: 6853448
  55. Products of nitrogen regulatory genes ntrA and ntrC of enteric bacteria activate glnA transcription in vitro: evidence that the ntrA product is a sigma factor.
    Proc Natl Acad Sci U S A. 1985 Nov;82(22):7525-9 PMID: 2999766
  56. Deduced product of the stage 0 sporulation gene spo0F shares homology with the Spo0A, OmpR, and SfrA proteins.
    Proc Natl Acad Sci U S A. 1985 Nov;82(21):7260-4 PMID: 2997779
  57. C(4)-dicarboxylate transport mutants of Rhizobium trifolii form ineffective nodules on Trifolium repens.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4284-8 PMID: 16593058
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.19.7921
Published
1987-10-12
Pages
7921-34
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306317
Subset
IM
Databases
GENBANK
X06253
External Links
PubMed source DOI: 10.1093/nar/15.19.7921 PMC Full Text View journal details
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