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PMID: 3671071 Published · ppublish English Journal Article

A long polypyrimidine/polypurine tract induces an altered DNA conformation on the 3' coding region of the adjacent myosin heavy chain gene.

Nucleic acids research ·Vol. 15 ·No. 19 ·1987-10-12 ·Pages 8069-85

McCarthy JG, Heywood SM

Abstract

A long (147 base pairs), natural A.T rich polypyrimidine/polypurine tract has been found 55 base pairs downstream of a chicken embryonic myosin heavy chain (MHC) gene. Analysis at the nucleotide level of nicks induced by S1 and Neurospora crassa nucleases indicate that this long interrupted polypyrimidine/polypurine tract exists in an alternate DNA structure in vitro at pH 4.5 and pH 7.5 in both supercoiled and linear plasmid DNA. The polypyrimidine/polypurine tract induces this alternate structure upon at least 200 base pairs of its 5' flanking DNA, and thus extends into the 3' coding and non-coding regions of the neighboring MHC gene. The different nicking patterns induced by the nucleases S1 and N. crassa on each strand of this alternate structure suggests that the polypyrimidine/polypurine tract may form heteronomous DNA. When this long polypyrimidine/polypurine tract is present in a supercoiled plasmid at low pH, a new and as yet undefined S1 hypersensitive DNA alteration was detected near the center of this tract.

MeSH Terms
Animals Base Sequence Chick Embryo DNA, Recombinant DNA, Superhelical Genes Molecular Sequence Data Myosins/genetics Nucleic Acid Conformation
Chemicals
DNA, Recombinant DNA, Superhelical Myosins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McCarthy J G
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06268.
Heywood S M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.19.8069
Published
1987-10-12
Pages
8069-85
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306327
Subset
IM
Databases
GENBANK
X06251
External Links
PubMed source DOI: 10.1093/nar/15.19.8069 PMC Full Text View journal details
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