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PMID: 368027 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt.

Journal of bacteriology ·Vol. 137 ·No. 1 ·1979-01-00 ·Pages 502-6

Thomson J, Gerstenberger PD, Goldberg DE, Gociar E, Orozco de Silva A, Fraenkel DG

Abstract

The Clarke-Carbon clone bank carrying ColE1-Escherichia coli DNA has been screened by conjugation for complementation of glycolysis and hexose monophosphate shunt mutations. Plasmids were identified for phosphofructokinase (pfkA), triose phosphate isomerase (tpi), phosphoglucose isomerase (pgi), glucose-6-phosphate dehydrogenase (zwf), gluconate-6-phosphate dehydrogenase (gnd), enolase (eno), phosphoglycerate kinase (pgk), and fructose-1,6-P2 aldolase (fda). Enzyme levels for the plasmid-carried gene ranged, for the various plasmids, from 4- to 25-fold the normal level.

MeSH Terms
Conjugation, Genetic DNA, Bacterial/genetics DNA, Recombinant Escherichia coli/genetics Genes Genetic Complementation Test Glycolysis Hexosephosphates/metabolism Mutation Plasmids
Chemicals
DNA, Bacterial DNA, Recombinant Hexosephosphates
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Thomson J
Gerstenberger P D
Goldberg D E
Gociar E
Orozco de Silva A
Fraenkel D G
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.137.1.502-506.1979
Published
1979-01-00
Pages
502-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218476
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.137.1.502-506.1979 PMC Full Text View journal details
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