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PMID: 3680372 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Drosophilia spectrin. I. Characterization of the purified protein.

The Journal of cell biology ·Vol. 105 ·No. 5 ·1987-11-00 ·Pages 2095-102

Dubreuil R, Byers TJ, Branton D, Goldstein LS, Kiehart DP

Abstract

We purified a protein from Drosophila S3 tissue culture cells that has many of the diagnostic features of spectrin from vertebrate organisms: (a) The protein consists of two equimolar subunits (Mr = 234 and 226 kD) that can be reversibly cross-linked into a complex composed of equal amounts of the two subunits. (b) Electron microscopy of the native molecule reveals two intertwined, elongated strands with a contour length of 180 nm. (c) Antibodies directed against vertebrate spectrin react with the Drosophila protein and, similarly, antibodies to the Drosophila protein react with vertebrate spectrins. One monoclonal antibody has been found to react with both of the Drosophila subunits and with both subunits of vertebrate brain spectrin. (d) The Drosophila protein exhibits both actin-binding and calcium-dependent calmodulin-binding activities. Based on the above criteria, this protein appears to be a bona fide member of the spectrin family of proteins.

MeSH Terms
Actins/metabolism Animals Antibodies Antibodies, Monoclonal Antigen-Antibody Complex Calmodulin/metabolism Cell Line Drosophila/metabolism Macromolecular Substances Microscopy, Electron Molecular Weight Spectrin/isolation & purification,metabolism
Chemicals
Actins Antibodies Antibodies, Monoclonal Antigen-Antibody Complex Calmodulin Macromolecular Substances Spectrin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dubreuil R
Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.
Byers T J
Branton D
Goldstein L S
Kiehart D P
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.105.5.2095
Published
1987-11-00
Pages
2095-102
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114846
Subset
IM
Grants
NIGMS NIH HHS · GM31579 · United States
NIGMS NIH HHS · GM33830 · United States
NIGMS NIH HHS · GM35252 · United States
External Links
PubMed source DOI: 10.1083/jcb.105.5.2095 PMC Full Text View journal details
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