Abstract
Urease activities of anaerobic bacteria that constituted predominant gut flora were examined. It was demonstrated that some strains of Eubacterium aerofaciens, E. lentum, and Peptostreptococcus products produced urease. They were the most numerous species in human feces. All strains of Bifidobacterium infantis and some strains of Bacteroides multiacidus, B. bifidum, Clostridium symbiosum, Fusobacterium necrophorum, F. varium, Lactobacillus fermentum, Peptococcus asaccharolyticus, and P. prevotii produced urease. The optimum pH of the Lactobacillus urease was found to be 4.0, whereas the pH value of B. multiacidus urease was 8.0.
MeSH Terms
Anaerobiosis
Bacteria/enzymology
Feces/microbiology
Humans
Hydrogen-Ion Concentration
Intestines/microbiology
Species Specificity
Urease/biosynthesis,metabolism
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Suzuki K
Benno Y
Mitsuoka T
Takebe S
Kobashi K
Hase J
References (16)
16 references, click to expand
-
Comparison of three procedures for biochemical testing of anaerobic bacteria.
J Clin Microbiol. 1975 Jan;1(1):15-24
PMID: 1176590
-
Hydrolysis of urea in the gastrointestinal tract of "monoxenic" rats: effect of immunization with strains of ureolytic bacteria.
Infect Immun. 1976 Jan;13(1):9-15
PMID: 1248879
-
The role of ammonia intoxication in hepatic coma.
Bull N Y Acad Med. 1958 Jun;34(6):357-65
PMID: 13536653
-
A MODIFIED TECHNIQUE FOR ULTRAMICRO ESTIMATIONS OF UREA NITROGEN.
Clin Chim Acta. 1963 Sep;8:810-2
PMID: 14069669
-
Anaerobiosis with iron wool.
Aust J Exp Biol Med Sci. 1955 Feb;33(1):33-7
PMID: 14389171
-
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75
PMID: 14907713
-
Isolation and characteristics of a ureolytic strain of Selenomonas ruminatium.
J Dairy Sci. 1974 Sep;57(9):1003-14
PMID: 4213200
-
Isolation of ureolytic Peptostreptococcus productus from feces using defined medium; failure of common urease tests.
Appl Microbiol. 1974 Oct;28(4):594-9
PMID: 4213672
-
Effect of diet on human fecal flora: comparison of Japanese and American diets.
Am J Clin Nutr. 1974 Dec;27(12):1456-69
PMID: 4432829
-
Human fecal flora: the normal flora of 20 Japanese-Hawaiians.
Appl Microbiol. 1974 May;27(5):961-79
PMID: 4598229
-
[Comparative studies on lactobacilli from the faeces of man, swine and chickens].
Zentralbl Bakteriol Orig. 1969 May;210(1):32-51
PMID: 4982872
-
Urea and amino acid supplementation of swine diets.
J Nutr. 1970 Mar;100(3):330-40
PMID: 5461704
-
Urea as a nitrogen source for pigs fed diets supplemented with lysine and methionine.
J Anim Sci. 1971 Jul;33(1):58-63
PMID: 5571090
-
[Improved methodology of qualitative and quantitative analysis of the intestinal flora of man and animals].
Zentralbl Bakteriol Orig. 1965 Mar;195(4):455-69
PMID: 5860078
-
Urease activity in the rumen of sheep and the isolation of ureolytic bacteria.
J Gen Microbiol. 1976 Jan;92(1):32-48
PMID: 812952
-
Urease assay and urease-producing species of anaerobes in the bovine rumen and human feces.
Appl Environ Microbiol. 1977 May;33(5):1097-104
PMID: 879770