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PMID: 3684578 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Primary structure of the DNA terminal protein of bacteriophage PRD1.

Nucleic acids research ·Vol. 15 ·No. 21 ·1987-11-11 ·Pages 8999-9009

Hsieh JC, Jung GH, Leavitt MC, Ito J

Abstract

The genome of a lipid-containing phage, PRD1, is replicated by a protein-priming mechanism. We have determined the nucleotide sequence of the PRD1 gene 8 which specifies the terminal protein, the protein primer for DNA synthesis. The coding region is 780 base pairs long and encodes for 259 amino acids (29,326 daltons). The predicted amino acid sequence of the PRD1 terminal protein reveals no substantial homology with that of any known terminal protein. However, hydropathy profiles of the PRD1, phi 29, and Nf terminal proteins are remarkably similar, suggesting a common evolutionary origin. A particular tyrosine residue is predicted to be covalently linked to the 5' end of the PRD1 DNA. The initiation codon ATG of gene 8 is preceded by the identifiable ribosome binding site, and putative promoter sequences. There are unique palindromic sequences between the ribosome binding site and "-10" region.

MeSH Terms
Amino Acid Sequence Bacteriophages/genetics Base Sequence DNA, Viral/genetics Molecular Sequence Data Viral Proteins/genetics
Chemicals
DNA terminal protein, Enterobacteria phage PRD1 DNA, Viral Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hsieh J C
Department of Microbiology and Immunology, College of Medicine, University of Arizona, Tucson 85724.
Jung G H
Leavitt M C
Ito J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.21.8999
Published
1987-11-11
Pages
8999-9009
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306418
Subset
IM
Grants
NIGMS NIH HHS · GM28013 · United States
Databases
GENBANK
X06321
External Links
PubMed source DOI: 10.1093/nar/15.21.8999 PMC Full Text View journal details
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