Home LiteratureArticle Details
PMID: 368568 Published · ppublish English Journal Article

An "up-promotor" mutation affecting the acetamidase of Aspergillus nidulans.

Molecular & general genetics : MGG ·Vol. 166 ·No. 1 ·1978-10-25 ·Pages 31-6

Hynes MJ

Abstract

A derivative of an areA200 strain of Aspergillus nidulans selected for strong growth on acetamide as the sole nitrogen source was found to have a mutation, amd-18, closely linked to amdS, the acetamidase structural gene. This mutation results in 2--3 fold higher acetamidase activities than wildtype strains in uninduced as well as induced cultures. The effects of the amd-18 mutation are superimposed on the effects of other regulatory mutations affecting the acetamidase. The amd-18 mutation is cis-dominant with respect to the amdS gene. Immunological and gel electrophoresis studies have shown that amd-18 strains produce higher levels of acetamidase protein. A previously described cis-dominant mutation, amdI9, also causes elevated levels of acetamidase protein. The amd-18 mutation may affect a promoter site for the amdS gene allowing an increased frequency of transcription.

MeSH Terms
Acetamides/metabolism Amidohydrolases/genetics Aspergillus nidulans/enzymology Genes Genetic Linkage Mutation Operon Phenotype
Chemicals
Acetamides Amidohydrolases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Hynes M J
References (30)
30 references, click to expand
  1. Induction of the acetamidase of Aspergillus nidulans by acetate metabolism.
    J Bacteriol. 1977 Sep;131(3):770-5 PMID: 19418
  2. Regulation of transcription termination in the leader region of the tryptophan operon of Escherichia coli involves tryptophan or its metabolic product.
    J Mol Biol. 1976 May 15;103(2):339-49 PMID: 781270
  3. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  4. The genetics of Aspergillus nidulans.
    Adv Genet. 1953;5:141-238 PMID: 13040135
  5. Initiator constitutive mutation with an 'up-promoter' effect in Aspergillus nidulans.
    Nature. 1975 Mar 6;254(5495):31-4 PMID: 1089904
  6. Cis-dominant regulatory mutations affecting the formation of glucose-repressible alcohol dehydrogenase (ADHII) in Saccharomyces cerevisiae.
    Mol Gen Genet. 1976 Jun 15;145(3):327-33 PMID: 781520
  7. Organization of the Rosy locus in Drosophila melanogaster.
    Cell. 1977 May;11(1):1-10 PMID: 406047
  8. Interaction of super-repressible and dominant constitutive mutations for the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae.
    Mol Gen Genet. 1977 Apr 29;152(3):137-44 PMID: 327270
  9. Characterization of a dominant, constitutive mutation, PHOO, for the repressible acid phosphatase synthesis in Saccharomyces cerevisiae.
    J Bacteriol. 1974 Nov;120(2):608-17 PMID: 4616940
  10. Nitrogen metabolite repression in Aspergillus nidulans.
    Mol Gen Genet. 1973 Nov 2;126(2):111-41 PMID: 4591376
  11. The genetic analysis of regulation of amidase synthesis in Aspergillus nidulans. II. Mutants resistant to fluoroacetamide.
    Mol Gen Genet. 1970;108(2):107-16 PMID: 4097637
  12. Multiple independent control mechanisms affecting the acetamidase of Aspergillus nidulans.
    Mol Gen Genet. 1978 Apr 25;161(1):59-65 PMID: 353500
  13. The genetic analysis of regulation of amidase synthesis in Aspergillus nidulans. I. Mutants able to utilize acrylamide.
    Mol Gen Genet. 1970;108(2):97-106 PMID: 5475570
  14. Mutants with altered glucose repression of amidase enzymes in Aspergillus nidulans.
    J Bacteriol. 1972 Sep;111(3):717-22 PMID: 4559822
  15. A cis-dominant regulatory mutation affecting enzyme induction in the eukaryote Aspergillus nidulans.
    Nature. 1975 Jan 17;253(5488):210-2 PMID: 1089206
  16. Regulation of genes controlling synthesis of the galactose pathway enzymes in yeast.
    Genetics. 1966 Sep;54(3):911-6 PMID: 5970626
  17. Temperature sensitive mutants affecting the activity and regulation of the acetamidase of Aspergillus nidulans.
    Mol Gen Genet. 1973 Jul 16;123(4):333-46 PMID: 4727141
  18. Linkage of genetic determinants for mouse beta-galactosidase electrophoresis and activity.
    Genetics. 1977 Jan;85(1):73-84 PMID: 838272
  19. Integrator gene in Aspergillus nidulans.
    Nature. 1976 Jul 15;262(5565):231-4 PMID: 778641
  20. Hyper-repressible operator-type mutant in sulphate permease gene of Aspergillus nidulans.
    Nature. 1976 Jan 29;259(5541):337-8 PMID: 765839
  21. Pleiotropic mutants of Aspergillus nidulans altered in carbon metabolism.
    Mol Gen Genet. 1977 Jan 18;150(2):193-204 PMID: 320455
  22. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  23. Studies on the role of the areA gene in the regulation of nitrogen catabolism in Aspergillus nidulans.
    Aust J Biol Sci. 1975 Jun;28(3):301-13 PMID: 52352
  24. Effects of ammonium, L-glutamate, and L-glutamine on nitrogen catabolism in Aspergillus nidulans.
    J Bacteriol. 1974 Dec;120(3):1116-23 PMID: 4612004
  25. A gene cluster in Aspergillus nidulans with an internally located cis-acting regulatory region.
    Nature. 1975 Mar 6;254(5495):26-31 PMID: 1089903
  26. Presumptive control mutation for alcohol dehydrogenase in Drosophila melanogaster.
    Nature. 1977 Nov 24;270(5635):363 PMID: 413052
  27. Analysis of bacteriophage T7 early RNAs and proteins on slab gels.
    J Mol Biol. 1973 Sep 15;79(2):237-48 PMID: 4760132
  28. Dual control for transcription of the galactose operon by cyclic AMP and its receptor protein at two interspersed promoters.
    Cell. 1977 Nov;12(3):847-54 PMID: 200371
  29. Regulation of arginine biosynthesis in Saccharomyces cerevisiae: isolation of a cis-dominant, constitutive mutant for ornithine carbamoyltransferase synthesis.
    J Bacteriol. 1976 Oct;128(1):49-55 PMID: 789352
  30. Increased and decreased sensitivity to carbon catabolite repression of enzymes of acetate metabolism in mutants of Aspergillus nidulans.
    Mol Gen Genet. 1977 Nov 4;156(1):87-92 PMID: 23491
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00379726
Published
1978-10-25
Pages
31-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00379726
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2