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PMID: 368655 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcription and processing of cloned yeast tyrosine tRNA genes microinjected into frog oocytes.

Nature ·Vol. 278 ·No. 5700 ·1979-03-08 ·Pages 137-43

De Robertis EM, Olson MV

Abstract

Xenopus oocytes transcribe cloned yeast tyrosine tRNA genes and process the RNA. The processing includes base modifications, addition of the CCA end, and splicing of the intervening sequence. The primary transcript has a 5' leader extension which varies considerably in seuqence and in length between different tRNATyr loci, but is nevertheless present in all of them.

MeSH Terms

Animals Base Sequence DNA, Recombinant DNA-Directed RNA Polymerases/metabolism Female Genes Nucleic Acid Precursors/genetics Oocytes/metabolism Plasmids RNA Ligase (ATP)/metabolism RNA, Transfer/genetics Ribonucleases/metabolism Saccharomyces cerevisiae/genetics Transcription, Genetic Tyrosine Xenopus/genetics

Chemicals

DNA, Recombinant Nucleic Acid Precursors Tyrosine RNA, Transfer DNA-Directed RNA Polymerases Ribonucleases RNA Ligase (ATP)

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

De Robertis E M

Olson M V

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/278137a0

Published

1979-03-08

Pages

137-43

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/278137a0 View journal details

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Transcription and processing of cloned yeast tyrosine tRNA genes microinjected into frog oocytes.

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