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PMID: 3691477 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Truncation does not abrogate transcriptional downregulation of the c-myc gene by sodium butyrate in Burkitt's lymphoma cells.

The EMBO journal ·Vol. 6 ·No. 10 ·1987-10-00 ·Pages 2959-64

Polack A, Eick D, Koch E, Bornkamm GW

Abstract

We have examined the effect of sodium butyrate, a potent inducer of differentiation in various cell systems, on the steady state RNA level and transcriptional activity of the c-myc gene in Burkitt's lymphoma cells. Following sodium butyrate treatment a rapid decrease of c-myc RNA was observed in all Burkitt's lymphoma cell lines studied, irrespective of the type of translocation, the location of the breakpoint relative to c-myc or of the association with EBV. Since cellular genes induced by interferon are suspected to play a role in c-myc regulation we have studied transcription of the 2-5A synthetase gene in sodium butyrate-treated Burkitt's lymphoma cells. Transcriptional activity and steady state mRNA levels of the 2-5A synthetase gene were induced by sodium butyrate. The time course of induction excluded, however, that the decrease of c-myc RNA is caused by induction of the 2-5A synthetase/RNase L endonuclease system. The reduction of c-myc RNA is caused, at least in part, by a reduced transcription rate, as shown by nuclear run-on analysis. The fact that sodium butyrate is capable of downregulating a truncated c-myc gene indicates that an important target site of transcriptional regulation is located outside the region encompassing the upstream regulatory sequences, the dual promoters and the leader region.

MeSH Terms
2',5'-Oligoadenylate Synthetase/genetics Burkitt Lymphoma/genetics Butyrates/pharmacology Butyric Acid Cell Line Gene Expression Regulation Genes Humans Nucleic Acid Hybridization Proto-Oncogenes/drug effects RNA, Messenger/genetics Transcription, Genetic/drug effects
Chemicals
Butyrates RNA, Messenger Butyric Acid 2',5'-Oligoadenylate Synthetase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Polack A
Abteilung für Virologie, Universität Freiburg, FRG.
Eick D
Koch E
Bornkamm G W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-10-00
Pages
2959-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553732
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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