Home LiteratureArticle Details
PMID: 3691735 Published · ppublish English Journal Article

Mechanical parameters determined in dispersed ventricular heart cells.

Experientia ·Vol. 43 ·No. 11-12 ·1987-12-01 ·Pages 1150-3

Niggli E

Abstract

A high-resolution laser diffraction system suitable for studying the basic mechanical properties of small contractile single cells has been developed. This method was used to establish the mechanical behavior of 95 ventricular cells isolated from adult guinea pig hearts. During contraction, the sarcomere length shortened from 1828 +/- 43 nm (mean +/- SD) to 1518 +/- 99 nm. The maximal velocities were 1.98 +/- 0.64 micron/s for shortening and 1.93 +/- 0.54 micron/s for re-lengthening. The twitch duration from 20% shortening to 80% re-lengthening was 622 +/- 120 ms.

MeSH Terms
Animals Calcium/pharmacology Electric Stimulation Guinea Pigs Heart Ventricles/ultrastructure Lasers Myocardial Contraction Sarcomeres/drug effects,ultrastructure Ventricular Function
Chemicals
Calcium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Niggli E
Department of Physiology, University of Bern, Switzerland.
References (13)
13 references, click to expand
  1. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
    Pflugers Arch. 1981 Aug;391(2):85-100 PMID: 6270629
  2. The effects of insulin and anoxia on the metabolism of isolated mature rat cardiac myocytes.
    Arch Biochem Biophys. 1980 Sep;203(2):587-99 PMID: 7006513
  3. A laser diffraction system with improved sensitivity for long-time measurements of sarcomere dynamics in isolated cardiac myocytes.
    Pflugers Arch. 1988 Apr;411(4):462-8 PMID: 3399366
  4. Uniform sarcomere shortening behavior in isolated cardiac muscle cells.
    J Gen Physiol. 1980 Nov;76(5):587-607 PMID: 7441197
  5. The two components in the shortening of unloaded ventricular myocytes: their voltage dependence.
    Basic Res Cardiol. 1985;80 Suppl 1:117-21 PMID: 2581544
  6. Analysis of the effects of changes in rate and rhythm upon electrical activity in the heart.
    Prog Biophys Mol Biol. 1980;36(1):1-52 PMID: 7001542
  7. Velocity of sarcomere shortening in rat cardiac muscle: relationship to force, sarcomere length, calcium and time.
    J Physiol. 1984 Oct;355:367-81 PMID: 6491996
  8. Influence of a change in stimulation rate on action potentials, currents and contractions in rat ventricular cells.
    J Physiol. 1985 Jul;364:113-30 PMID: 2411913
  9. Electrophysiology of single cardiac cells.
    Jpn J Physiol. 1984;34(3):375-88 PMID: 6092750
  10. Cell pairs isolated from adult guinea pig and rat hearts: effects of [Ca2+]i on nexal membrane resistance.
    Pflugers Arch. 1987 Aug;409(4-5):394-402 PMID: 3627957
  11. Myocardial sarcomere dynamics during isometric contraction.
    J Physiol. 1975 Oct;251(3):627-43 PMID: 1185677
  12. Contraction in voltage-clamped, internally perfused single heart cells.
    J Gen Physiol. 1986 Oct;88(4):475-505 PMID: 2431095
  13. Sarcomere dynamics in intact cardiac muscle.
    Eur J Cardiol. 1976 May;4 Suppl:53-65 PMID: 1278219
Article Info
Journal
Experientia
Abbr.
Experientia
ISSN
0014-4754
DOI
10.1007/BF01945514
Published
1987-12-01
Pages
1150-3
Language
English
Region
Switzerland
NLM ID
0376547
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01945514
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2