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PMID: 3693399 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mobility of filamentous actin in living cytoplasm.

The Journal of cell biology ·Vol. 105 ·No. 6 Pt 1 ·1987-12-00 ·Pages 2811-6

Wang YL

Abstract

Filamentous actin in living cultured cells was labeled by microinjecting trace amounts of rhodamine-phalloidin (rh-pha) as a specific, high-affinity probe. The microinjection caused no detectable effect on cell morphology or cell division. The distribution of rh-pha-labeled filaments was then examined in dividing cells using image-intensified fluorescence microscopy, and the exchangeability of labeled filaments along stress fibers was studied during interphase using fluorescence recovery after photobleaching. rh-pha showed a rapid concentration at the contractile ring during cell division. In addition, recovery of fluorescence after photobleaching occurred along stress fibers with a halftime as short as 8 min. These observations suggest that at least some actin filaments undergo continuous movement and reorganization in living cells. This dynamic process may play an important role in various cellular functions.

MeSH Terms
Actins/metabolism Animals Cell Division Cells, Cultured Cytoplasm/metabolism,ultrastructure Fluorescent Dyes Mice Microscopy, Fluorescence
Chemicals
Actins Fluorescent Dyes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Wang Y L
Doris W. Neustadt Laboratory of Cellular Structure, Department of Molecular and Cellular Biology, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.105.6.2811
Published
1987-12-00
Pages
2811-6
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114689
Subset
IM
Grants
NIGMS NIH HHS · GM-32476 · United States
External Links
PubMed source DOI: 10.1083/jcb.105.6.2811 PMC Full Text View journal details
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