Separation of cryptosporidium oocysts from fecal debris by density gradient centrifugation and glass bead columns.

Journal of clinical microbiology ·Vol. 23 ·No. 4 ·1986-04-00 ·Pages 789-91

Heyman MB, Shigekuni LK, Ammann AJ

Abstract

A method was developed to obtain purified cryptosporidium oocysts from fecal samples. Oocysts were initially collected by centrifugation through a sucrose density gradient and further purified by passage through glass bead columns. The purified oocysts were antigenically active and sufficiently pure for immunological studies.

MeSH Terms

Animals Antigens, Protozoan/immunology Cattle Centrifugation, Density Gradient Coccidia/isolation & purification Cryptosporidium/immunology,isolation & purification Feces/parasitology Humans

Chemicals

Antigens, Protozoan

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Heyman M B

Shigekuni L K

Ammann A J

References (6)

6 references, click to expand

Purification of Eimeria tenella sporozoites with glass bead columns.
J Parasitol. 1969 Aug;55(4):833-8 PMID: 5822644
Experimental cryptosporidiosis in laboratory mice.
Infect Immun. 1982 Nov;38(2):471-5 PMID: 7141705
A cleaning method for coccidial oocysts using density-gradient sedimentation.
J Parasitol. 1963 Feb;49:159-60 PMID: 13976882
The species of Cryptosporidium (Apicomplexa: Cryptosporidiidae) infecting mammals.
J Parasitol. 1985 Oct;71(5):625-9 PMID: 4057006
Separation of nematode eggs from fecal debris by gradient centrifugation.
J Parasitol. 1961 Apr;47:248-50 PMID: 13767065
Techniques for the recovery and identification of Cryptosporidium oocysts from stool specimens.
J Clin Microbiol. 1983 Jul;18(1):185-90 PMID: 6193138

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.23.4.789-791.1986

Published

1986-04-00

Pages

789-91

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC362839

Subset

External Links

PubMed source DOI: 10.1128/jcm.23.4.789-791.1986 PMC Full Text View journal details

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Separation of cryptosporidium oocysts from fecal debris by density gradient centrifugation and glass bead columns.

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