Home LiteratureArticle Details
PMID: 370553 Published · ppublish English Journal Article Review

Staphylococcal nuclease reviewed: a prototypic study in contemporary enzymology. I. Isolation; physical and enzymatic properties.

Molecular and cellular biochemistry ·Vol. 22 ·No. 2-3 ·1978-12-22 ·Pages 67-77

Tucker PW, Hazen EE, Cotton FA

Abstract

This is the first of a series of four articles in which the chemical, enzymological, and crystallographic work on Ribonucleate (deoxyribonucleate)-3'-nucleotidohydrolase, EC 3.1.4.7, (Staphylococcal nuclease, Micrococcal nuclease) will be reviewed and correlated. This article discusses the purification of the enzyme and its general physical and enzymological properties. Subsequent articles will deal with specific studies of the nucleotide binding site, crystallographic studies of a nuclease-inhibitor complex, use of the nuclease as a model for protein folding and possible mechanisms for the action of the enzyme.

MeSH Terms
Amino Acid Sequence Calcium/pharmacology Kinetics Micrococcal Nuclease/isolation & purification,metabolism Molecular Weight Substrate Specificity
Chemicals
Micrococcal Nuclease Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tucker P W
Hazen E E
Cotton F A
References (63)
63 references, click to expand
  1. Action of venom phosphodiesterase on deoxyribonucleic acid.
    J Biol Chem. 1961 Apr;236:1130-4 PMID: 13785422
  2. Concentration and desalting of ribonucleases.
    Biochem Biophys Res Commun. 1963 Feb 18;10:311-4 PMID: 13975595
  3. Principles that govern the folding of protein chains.
    Science. 1973 Jul 20;181(4096):223-30 PMID: 4124164
  4. The amino acid sequence of an extracellular nuclease of Staphylococcus aureus. II. The amino acid sequences of tryptic and chymotryptic peptides.
    J Biol Chem. 1967 Oct 25;242(20):4736-51 PMID: 4294023
  5. Crystalline extracellular nuclease of Staphylococcus aureus.
    J Biol Chem. 1966 Oct 10;241(19):4389-90 PMID: 5922963
  6. Synthesis of staphylococcal enterotoxin A and nuclease under controlled fermentor conditions.
    Appl Environ Microbiol. 1976 Feb;31(2):243-8 PMID: 11744
  7. Crystalline desoxyribonuclease; digestion of thymus nucleic acid; the kinetics of the reaction.
    J Gen Physiol. 1950 Mar;33(4):363-77 PMID: 15406374
  8. The structural basis of the catalytic function of staphylococcal nuclease.
    Brookhaven Symp Biol. 1968 Jun;21(1):172-200 PMID: 4305102
  9. Staphylococcal nuclease (Foggi strain). I. Order of cyanogen bromide fragments and a "fourth" histidine residue.
    J Biol Chem. 1968 Sep 25;243(18):4769-77 PMID: 5687719
  10. THE USE OF PURIFIED MICROCOCCAL NUCLEASE IN IDENTIFYING THE NUCLEOTIDE TERMINUS BEARING A FREE 5'-MONOPHOSPHATE.
    J Biol Chem. 1964 Oct;239:3498-504 PMID: 14245408
  11. The effect of urea on staphylococcal nuclease digestion of chromatin.
    Biochem Biophys Res Commun. 1975 Dec 15;67(4):1391-400 PMID: 1201093
  12. DNases and their use in the studies of primary structure of nucleic acids.
    Adv Enzymol Relat Areas Mol Biol. 1967;29:165-220 PMID: 4882960
  13. The amino acid sequence of an extracellular nuclease of Staphylococcus aureus. I. Linear order of the fragments produced by cleavage with cyanogen bromide.
    J Biol Chem. 1966 Oct 10;241(19):4366-85 PMID: 5922961
  14. Quantitative affinity chromatography. Determination of binding constants by elution with competitive inhibitors.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2382-5 PMID: 4526212
  15. Susceptibility of dinucleotides bearing either 3'- or 5'-monophosphate to micrococcal nuclease.
    J Biol Chem. 1969 Dec 25;244(24):6559-65 PMID: 4312002
  16. Subunit structure of chromatin.
    Nature. 1974 Sep 20;251(5472):249-51 PMID: 4422492
  17. Deoxyribonuclease activity of micrococci from clinical sources.
    J Bacteriol. 1957 Jun;73(6):747-53 PMID: 13449041
  18. Separable forms of nuclease secreted by Staphylococcus aureus as a function of growth stage.
    Can J Microbiol. 1974 Mar;20(3):337-45 PMID: 4822052
  19. The large scale preparation of an extracellular nuclease of Staphylococcus aureus.
    J Biol Chem. 1969 Jan 25;244(2):497-9 PMID: 5773310
  20. Degradation of tobacco mosiac virus nucleic acid with micrococcal phosphodiesterase.
    Biochim Biophys Acta. 1959 Nov;36:132-42 PMID: 14436791
  21. Mechanism of action of micrococcal nuclease on deoxyribonucleic acid.
    J Biol Chem. 1962 Aug;237:2620-5 PMID: 13918300
  22. Selective enzyme purification by affinity chromatography.
    Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43 PMID: 4971842
  23. Purification and some enzymic properties of Staphylococcus aureus deoxyribonuclease.
    Biochim Biophys Acta. 1966 Sep;123(3):493-502 PMID: 5971435
  24. Nuclease-T: an active derivative of staphylococcal nuclease composed of two noncovalently bonded peptide fragments.
    Proc Natl Acad Sci U S A. 1967 Sep;58(3):1235-42 PMID: 5233844
  25. Action of micrococcal phosphodiesterase on tobacco mosaic virus nucleic acid.
    Nature. 1958 Nov 8;182(4645):1308 PMID: 13600307
  26. The interaction of the lanthanide ions with staphylococcal nuclease.
    J Biol Chem. 1973 Aug 25;248(16):2821-5 PMID: 4723899
  27. Hydrolysis of ribo- and deoxyribo-dinucleotides by micrococcal nuclease.
    Biochim Biophys Acta. 1970 Oct 15;217(2):538-40 PMID: 5473202
  28. [Structure of oligonucleotides liberated by the action of desoxyribonuclease on Staphylococcus pyogenes].
    Bull Soc Chim Biol (Paris). 1960;42:795-815 PMID: 13736435
  29. Mode of action of micrococcal phosphodiesterase.
    Nature. 1960 Jul 2;187:74-5 PMID: 14436792
  30. An improved method for the purification of staphylococcal nuclease.
    J Biol Chem. 1967 Oct 25;242(20):4768-70 PMID: 4294026
  31. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.
    Biochem Biophys Res Commun. 1973 May 15;52(2):504-10 PMID: 4711166
  32. Studies on isomeric dinucleotides derived from deoxyribonucleic acid.
    J Biol Chem. 1957 Feb;224(2):751-8 PMID: 13405903
  33. Action of micrococcal nuclease on polymers of deoxyadenylic and deoxythymidylic acids.
    J Biol Chem. 1969 Jul 25;244(14):3818-22 PMID: 4308736
  34. MICROCOCCAL NUCLEASE AS A PROBE OF DNA CONFORMATION.
    Biochemistry. 1964 Jan;3:27-39 PMID: 14114500
  35. Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus.
    J Biol Chem. 1967 Apr 10;242(7):1541-7 PMID: 4290246
  36. Polyamine activation of staphylococcal nuclease.
    Biochim Biophys Acta. 1975 Apr 16;390(1):117-24 PMID: 236039
  37. Steps in the formation of active derivatives of staphylococcal nuclease during trypsin digestion.
    J Biol Chem. 1968 Sep 25;243(18):4778-86 PMID: 5687720
  38. Studies on the mechanism of action of micrococcal nuclease. 1. Degradation of thymus deoxyribonucleic acid.
    Biochim Biophys Acta. 1962 May 14;55:664-73 PMID: 14492805
  39. The occurrence of autoacceleration during hydrolysis of nucleic acids by micrococcal nuclease.
    J Biol Chem. 1968 Sep 25;243(18):4917-21 PMID: 4301228
  40. Specific sites of interaction between histones and DNA in chromatin.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4101-5 PMID: 4530287
  41. Studies of extracellular and intracellular bacterial deoxyribonucleic acids.
    J Gen Microbiol. 1958 Dec;19(3):522-39 PMID: 13611195
  42. The mechanism of stabilization of the structure of nuclease-T by binding of ligands.
    J Biol Chem. 1975 Mar 25;250(6):2388-94 PMID: 163827
  43. Use of micrococcal nuclease to monitor hybridization reactions with DNA.
    Anal Biochem. 1974 Apr;58(2):534-40 PMID: 4597219
  44. Characterization of a nuclease produced by Staphylococcus aureus.
    J Biol Chem. 1967 Mar 10;242(5):1016-20 PMID: 6020427
  45. The pH dependence of the steady state kinetic parameters for staphylococcal nuclease-catalyzed hydrolysis of deoxythymidine-3'-phosphate-5'-p-nitrophenylphosphate in H 2 O and D 2 O.
    J Biol Chem. 1973 Jul 10;248(13):4769-74 PMID: 4352408
  46. Phosphatase-free crystalline micrococcal nuclease.
    J Biol Chem. 1966 Oct 10;241(19):4386-8 PMID: 5922962
  47. A comparison of the digestion of nuclei and chromatin by staphylococcal nuclease.
    Biochemistry. 1975 Jul;14(13):2915-20 PMID: 1148184
  48. Cleavage point during hydrolyses catalyzed by ribonuclease and phosphodiesterase.
    Biochim Biophys Acta. 1961 Oct 14;53:214-5 PMID: 13907471
  49. [Purification of the deoxyribonuclease from Micrococcus pyogenes].
    Bull Soc Chim Biol (Paris). 1958;40(12):1905-10 PMID: 13629231
  50. Nuclear magnetic resonance studies of the structure and binding sites of enzymes. I. Histidine residues.
    Proc Natl Acad Sci U S A. 1967 Oct;58(4):1307-13 PMID: 5237865
  51. The amino acid sequence of an extracellular nuclease of Staphylococcus aureus. 3. Complete amino acid sequence.
    J Biol Chem. 1967 Oct 25;242(20):4752-8 PMID: 4294024
  52. Micrococcal nuclease and some products of its action.
    Ann N Y Acad Sci. 1959 Sep 4;81:788-91 PMID: 13813313
  53. The purification and properties of micrococcal nuclease.
    J Biol Chem. 1961 Nov;236:3014-9 PMID: 13860347
  54. SOME NEW APPLICATIONS OF MICROCOCCAL NUCLEASE TO THE ELUCIDATION OF THE STRUCTURE OF NUCLEIC ACIDS.
    Biochim Biophys Acta. 1964 Nov 15;91:433-45 PMID: 14254016
  55. Inhibition of micrococcal nuclease with 5'-deoxythymidine-5'-phosphonic acid containing oligomers.
    Biochemistry. 1972 Jan 4;11(1):9-12 PMID: 4333199
  56. The action of staphylococcal nuclease on synthetic substrates.
    Biochemistry. 1969 Jun;8(6):2277-84 PMID: 4307992
  57. Isolation of mutants of Staphylococcus aureus lacking extracellular nuclease activity.
    J Bacteriol. 1970 Mar;101(3):921-4 PMID: 4985592
  58. Action of micrococcal nuclease on chemically modified deoxyribonucleic acid.
    Biochemistry. 1974 Mar 12;13(6):1082-7 PMID: 4360774
  59. Degradation of thymus DNA and crab poly d(A-T) by micrococcal nuclease in the presence of actinomycin D.
    Biochim Biophys Acta. 1968 Mar 18;157(1):207-9 PMID: 4296061
  60. Chromatin structure: a repeating unit of histones and DNA.
    Science. 1974 May 24;184(4139):868-71 PMID: 4825889
  61. Staphylococcal nuclease (Foggi strain). II. The amino acid sequence.
    J Biol Chem. 1971 May 25;246(10):3103-10 PMID: 4324890
  62. FURTHER PURIFICATION OF MICROCOCCAL NUCLEASE FROM THE STRAIN SA-B.
    Biochem Biophys Res Commun. 1965 Jan 4;18:136-40 PMID: 14265746
  63. The binding of nucleotides and calcium to the extracellular nuclease of Staphylococcus aureus. Studies by gel filtration.
    J Biol Chem. 1967 Jul 10;242(13):3063-7 PMID: 4291070
Article Info
Journal
Molecular and cellular biochemistry
Abbr.
Mol Cell Biochem
ISSN
0300-8177
DOI
10.1007/BF00496235
Published
1978-12-22
Pages
67-77
Language
English
Region
Netherlands
NLM ID
0364456
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00496235 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2