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PMID: 3707539 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Kinetics of metabolic pathways. A system in vitro to study the control of flux.

The Biochemical journal ·Vol. 234 ·No. 1 ·1986-02-15 ·Pages 169-74

Torres NV, Mateo F, Meléndez-Hevia E, Kacser H

Abstract

A method for determining Control Coefficients is proposed for systems studied in vitro and applied to a model pathway. Rat liver extract, which converts glucose into glycerol 3-phosphate, was used with the addition to the incubation mixture of fructose-bisphosphate aldolase, triose-phosphate isomerase and glycerol-3-phosphate dehydrogenase as 'auxiliary' enzymes, which leaves all the control on the first three enzymes. The flux of the metabolic pathway was recorded by assaying NADH decay. Flux Control Coefficients (CJE) of hexokinase, glucose-6-phosphate isomerase and phosphofructokinase were calculated by titration of the system with increasing quantities of extraneous enzymes. It is shown that the summation property is fulfilled. The applicability of this procedure to study the control in any metabolic pathway is discussed. Possible relevance of the method to conditions in vivo and its limitations are considered.

MeSH Terms

Animals Female Glucose/metabolism Glycerophosphates/metabolism In Vitro Techniques Kinetics Liver/enzymology,metabolism Metabolism Methods Models, Biological Rats Rats, Inbred Strains

Chemicals

Glycerophosphates alpha-glycerophosphoric acid Glucose

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Torres N V

Mateo F

Meléndez-Hevia E

Kacser H

References (26)

26 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2340169

Published

1986-02-15

Pages

169-74

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1146541

Subset

External Links

PubMed source DOI: 10.1042/bj2340169 PMC Full Text View journal details

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Kinetics of metabolic pathways. A system in vitro to study the control of flux.

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