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PMID: 370779 Published · ppublish English Journal Article

2'-Deoxy-2'-fluorouridine-5'-phosphate: an alternative substrate for thymidylate synthetase from Escherichia coli K12.

Nucleic acids research ·Vol. 5 ·No. 12 ·1978-12-00 ·Pages 4753-9

Wohlrab F, Haertlé T, Trichtinger T, Guschlbauer W

Abstract

The substrate specificity of 2'-deoxy-2'-substituted uridines and their 5'-phosphates towards thymidylate synthetase from Escherichia coli K12 was investigated. Besides the natural substrate 2'-deoxyuridine-5'-phosphate (dUMP), only 2'-deoxy-2'-fluorouridine-5'-phosphate (dUflMP) was a substrate. The KM of dUflMP is 11 times higher than that of dUMP, while the Vmax values are virtually the same. It is concluded that the size of the 2'-substituent and not its polarity (and the concomitant conformational change) determines substrate specificity of thymidylate synthetase.

MeSH Terms
Deoxyuracil Nucleotides Escherichia coli/enzymology Fluorodeoxyuridylate Kinetics Methyltransferases/metabolism Molecular Conformation Substrate Specificity Thymidylate Synthase/metabolism
Chemicals
Deoxyuracil Nucleotides Fluorodeoxyuridylate Methyltransferases Thymidylate Synthase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wohlrab F
Haertlé T
Trichtinger T
Guschlbauer W
References (28)
28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.12.4753
Published
1978-12-00
Pages
4753-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342786
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.12.4753 PMC Full Text View journal details
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