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PMID: 3714487 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance.

Nucleic acids research ·Vol. 14 ·No. 11 ·1986-06-11 ·Pages 4617-24

Vara JA, Portela A, Ortín J, Jiménez A

Abstract

The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromycin N-acetyl transferase gene has been used as a dominant marker for the selection of transformed mammalian cells able to grow in the presence of the antibiotic.

MeSH Terms
Acetyltransferases/genetics Animals Chlorocebus aethiops Cloning, Molecular Drug Resistance Gene Expression Regulation Genes, Bacterial Genetic Vectors Puromycin/pharmacology Streptomyces/genetics Transfection
Chemicals
Puromycin Acetyltransferases puromycin N-acetyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vara J A
Portela A
Ortín J
Jiménez A
References (18)
18 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.11.4617
Published
1986-06-11
Pages
4617-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311469
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.11.4617 PMC Full Text View journal details
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