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PMID: 372 Published · ppublish English Journal Article

Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures.

Journal of bacteriology ·Vol. 124 ·No. 3 ·1975-12-00 ·Pages 1604-6

Cabib E, Duran A

Abstract

After mutagenesis, surviving yeast cells are grown on plates at 25 C and later exposed to 37 C. The plates are then overlaid with a soft agar containing p-nitrophenylphosphate at pH 9.7. Lysed cells liberate alkaline phosphatase which gives rise to a yellow color on and around colonies.

MeSH Terms
Agar Alkaline Phosphatase/metabolism Ethyl Methanesulfonate Hydrogen-Ion Concentration Mutagens Mutation Nitrophenols Organophosphorus Compounds Saccharomyces cerevisiae/enzymology,growth & development,isolation & purification Temperature
Chemicals
Mutagens Nitrophenols Organophosphorus Compounds Agar Ethyl Methanesulfonate Alkaline Phosphatase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cabib E
Duran A
References (4)
4 references, click to expand
  1. Changes in the phosphatase activity of Baker's yeast during the growth phase and location of the phosphatases in the yeast cell.
    Biochim Biophys Acta. 1960 Jan 29;37:482-90 PMID: 13835748
  2. The acid phosphatase of yeast. Localization and secretion by protoplasts.
    Biochim Biophys Acta. 1963 Feb 12;67:324-6 PMID: 13932174
  3. GENETIC ANALYSIS OF THE PHOSPHATASES IN ASPERGILLUS NIDULANS.
    Genet Res. 1965 Feb;6:13-26 PMID: 14301537
  4. High resolution colony staining for the detection of bacterial growth requirement mutants using naphthol azo-dye techniques.
    Biochem Biophys Res Commun. 1965 Oct 26;21(2):182-6 PMID: 4159772
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.124.3.1604-1606.1975
Published
1975-12-00
Pages
1604-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC236077
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.124.3.1604-1606.1975 PMC Full Text View journal details
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