Solid-phase microtiter radioimmunoassay blocking test for detection of antibodies to Escherichia coli heat-labile enterotoxin.

Journal of clinical microbiology ·Vol. 9 ·No. 1 ·1979-01-00 ·Pages 60-4

Greenberg HB, Levine MM, Merson MH, Sack RB, Sack DA, Valdesuso JR, Nalin D, Hoover D, Chanock RM, Kapikian AZ

Abstract

The development of a solid-phase microtiter radioimmunoassay blocking test to detect serum antibody to Escherichia coli heat-labile enterotoxin is described. The assay is easy to perform and quantitate, and it is sensitive and specific.

MeSH Terms

Adult Antibodies/analysis Diarrhea/immunology Drug Stability Enterotoxins/immunology Escherichia coli/immunology Escherichia coli Infections/immunology Hot Temperature Humans Neutralization Tests Radioimmunoassay/methods

Chemicals

Antibodies Enterotoxins

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Greenberg H B

Levine M M

Merson M H

Sack R B

Sack D A

Valdesuso J R

Nalin D

Hoover D

Chanock R M

Kapikian A Z

References (14)

14 references, click to expand

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J Clin Microbiol. 1977 Jun;5(6):570-2 PMID: 69630
Enterotoxigenic Escherichia coli diarrhea of travelers: a prospective study of American Peace Corps volunteers.
Johns Hopkins Med J. 1977 Aug;141(2):63-70 PMID: 196131
Microtiter solid-phase radioimmunoassay for detection of Escherichia coli heat-labile enterotoxin.
Infect Immun. 1977 Sep;17(3):541-5 PMID: 332626
Humoral immune response to the heat-labile enterotoxin of Escherichia coli in naturally acquired diarrhea and antitoxin determination by passive immune hemolysis.
Infect Immun. 1977 Jun;16(3):781-8 PMID: 330395
Microtiter solid-phase radioimmunoassay for hepatitis B antigen.
Appl Microbiol. 1973 Oct;26(4):478-84 PMID: 4201649
Antitoxin responses to infections with enterotoxigenic Escherichia coli.
J Infect Dis. 1974 Mar;129(3):330-5 PMID: 4593243
Immunologic cross-reactions of enterotoxins from Escherichia coli and Vibrio cholerae.
J Infect Dis. 1973 Feb;127(2):164-70 PMID: 4570128
Tissue-culture assay of antibodies to heat-liable Escherichia coli enterotoxins.
N Engl J Med. 1974 Jul 18;291(3):117-21 PMID: 4600168
Human reovirus-like agent as the major pathogen associated with "winter" gastroenteritis in hospitalized infants and young children.
N Engl J Med. 1976 Apr 29;294(18):965-72 PMID: 176586
Human diarrheal disease caused by enterotoxigenic Escherichia coli.
Annu Rev Microbiol. 1975;29:333-53 PMID: 1101807
Antibodies to heat-labile Escherichia coli enterotoxin in Apaches in Whiteriver, Arizona.
Infect Immun. 1975 Dec;12(6):1475-7 PMID: 1107221
Challenge of dogs with live enterotoxigenic Escherichia coli and effects of repeated challenges on fluid secretion in jejunal Thiry-Vella loops.
J Infect Dis. 1976 Jul;134(1):15-24 PMID: 781145
Travelers' diarrhea in Mexico. A prospective study of physicians and family members attending a congress.
N Engl J Med. 1976 Jun 10;294(24):1299-305 PMID: 772435
Transmission of acute infectious nonbacterial gastroenteritis to volunteers by oral administration of stool filtrates.
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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.9.1.60-64.1979

Published

1979-01-00

Pages

60-4

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC272957

Subset

External Links

PubMed source DOI: 10.1128/jcm.9.1.60-64.1979 PMC Full Text View journal details

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Solid-phase microtiter radioimmunoassay blocking test for detection of antibodies to Escherichia coli heat-labile enterotoxin.

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