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PMID: 3725591 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Variations in duplex DNA conformation detected by the binding of monoclonal autoimmune antibodies.

Nucleic acids research ·Vol. 14 ·No. 12 ·1986-06-25 ·Pages 5049-65

Braun RP, Lee JS

Abstract

Four monoclonal antibodies (Jel 229, 239, 241, 242) which bound to duplex DNA were prepared from two autoimmune female NZB/NZW mice. Their binding to various nucleic acids was investigated by a competitive solid phase radioimmune assay which allows the estimation of relative binding constants. None of the antibodies showed any consistent variation of binding constant with base composition and thus they must recognize features of the DNA backbone. Jel 241 binds across the major groove but the interaction with poly(pyrimidine) X poly(purine) DNAs was barely detectable. This antibody appears to recognize the "alternating-B" conformation which is promoted by methylation of pyrimidines in alternating sequences. The other three antibodies bind in the minor groove. In particular, for Jel 229 the preferred antigen was poly(dG) X poly(dC) with only weak binding to poly(dA) X poly(dT). This suggests a requirement for a wide minor groove. Thus autoimmune antibodies provide examples of "analogue" recognition and can be used to detect structural variations in the grooves of duplex DNA.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antibody Affinity Antibody Specificity Autoimmune Diseases/immunology Base Sequence DNA/immunology DNA-Binding Proteins/metabolism Mice Mice, Inbred NZB Molecular Weight Nucleic Acid Conformation Oligodeoxyribonucleotides/chemical synthesis,immunology Structure-Activity Relationship
Chemicals
Antibodies, Monoclonal DNA-Binding Proteins Oligodeoxyribonucleotides DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Braun R P
Lee J S
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39 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.12.5049
Published
1986-06-25
Pages
5049-65
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311510
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.12.5049 PMC Full Text View journal details
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